Di-Methyl-Histone H3 Antibodies for Dot Blot

2 matching Di-Methyl-Histone H3 antibodies for Dot Blot after applying filters

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Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. A Dot Blot analysis was performed to test the cross reactivity of the anti-H3K9me2 antibody (Product # 49-1007) with peptides containing other modifications of histone H3 and the unmodified H3K9 sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:20,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1013

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. A dot blot analysis was performed to test the cross reactivity of the anti-H3K27me2 (Product # 49-1013) with other histone H3 modifications. Other H3 modifications include mono- and trimethylation of the same lysine and mono-, di-, and trimethylation of lysine 9, 36, and 79. 100 to 0.2 pmol of peptide containing the respective histone modification was spotted on a membrane and detected with the antibody diluted 1:20,000. This figure shows a high specificity of the antibody for the modification o

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. A dot blot analysis was performed to test the cross reactivity of the anti-H3R17me2 crude serum (Product # 49-1021) with other modifications of histone H3 and H4 and with the unmodified histone H3. 100 to 0.2 pmol of each of the peptides containing the histone modifications was spotted on a membrane. The antibody was used at a dilution of 1:20,000. This figure shows a high specificity of the crude serum for the modification of interest.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. A dot blot analysis was performed to test the cross reactivity of the anti-H3K79me2 crude serum (Product # 49-1019) with other modifications of histone H3. Other H3 modifications include mono- and trimethylation of the same lysine and mono-, di-, and trimethylation of lysine 9, 27, and 36. 100 to 0.2 pmol of the peptide containing the respective histone modification was spotted on a membrane. The antibody was used at a dilution of 1:50,000. This figure shows a high specificity of the crude serum

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. A dot blot analysis was performed to test the cross reactivity of the anti-H3K4me2 crude serum (Product # 49-1004) with other modifications of histone H3 and with the unmodified protein. Other histone modifications include mono- and trimethylation of the same lysine and mono-, di-, and trimethylation of lysine 9, 27, 36, and 79. 100 to 0.2 pmol of the peptide containing the respective histone modification or the unmodified peptide were spotted on a membrane. The antibody was used at a dilution o

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 720092

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies. The specificity of the Antibody, Anti-Histone H3K9me2 Rabbit Polyclonal Antibody (Product # 720092, 1-2 ug/ml) to Histone H3K9me2 peptide was confirmed using MODified™ Histone Peptide Array (Product #13001, Active Motif). Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 ug/ml, 1:2500 dilution) was used as secondary Antibody. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Produc

Rabbit Poly, 100 ug
Hu
ChIP, DB, FACS, ICC, IF, WB

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-24678 Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies. A peptide dotblot shows Di-Methyl-Histone H3 (Lys23) Antibody (Product # MA5-24678) reacts only to Histone H3 Dimethyl-Lysine 23 (K23me2).

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies. A peptide dotblot shows Di-Methyl-Histone H3 (Lys23) Antibody (Product # MA5-24678) reacts only to Histone H3 Dimethyl-Lysine 23 (K23me2).

Rabbit Mono [RM171], 100 ug
Hu
AP, ChIP, DB, ELISA, IF, WB

AP,

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP, ELISA, Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-24677 Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies. A peptide dotblot shows Di-Methyl-Histone H3 (Lys18) Antibody (Product # MA5-24677) reacts only to Histone H3 Dimethyl-Lysine 18 (K18me2).

Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies. A peptide dotblot shows Di-Methyl-Histone H3 (Lys18) Antibody (Product # MA5-24677) reacts only to Histone H3 Dimethyl-Lysine 18 (K18me2).

Rabbit Mono [RM168], 100 ug
Hu
AP, DB, ELISA, IF, WB

AP, ELISA, Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies, Immunofluorescence image

IF,

Western Blot

Supplier data
Invitrogen Antibodies 49-1007

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Dot Blot image

DB,

ELISA,

IF,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1013 Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. Histone extracts (10 µg) from NB4 cells were analyzed by western blot using anti-H3K27me2 antibody (Product # 49-1013), diluted 1:500 in TBS-Tween. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. Histone extracts (10 µg) from NB4 cells were analyzed by western blot using anti-H3K27me2 antibody (Product # 49-1013), diluted 1:500 in TBS-Tween. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Dot Blot image

DB,

ELISA,

IF,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1021 Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3R17me2 crude serum (Product # 49-1021), diluted 1:250 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3R17me2 crude serum (Product # 49-1021), diluted 1:250 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Dot Blot image

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1019 Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K79me2 crude serum (Product # 49-1019), diluted 1:250 in TBS-Tween containing 5% milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K79me2 crude serum (Product # 49-1019), diluted 1:250 in TBS-Tween containing 5% milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Dot Blot image

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1004 Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K4me2 crude serum (Product # 49-1004), diluted 1:750 in TBS-Tween containing 5% skim milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K4me2 crude serum (Product # 49-1004), diluted 1:750 in TBS-Tween containing 5% skim milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Dot Blot image

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 720092

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies. Western blot analysis of Histone H3K9me2 was performed by loading 1 µg of from MCF7 (lane 1), MDA-MB -231 (lane 2) and MDA-MB-468 (lane 3) HCl extracted histones and 1 µg of HeLa oligo-nucleosomes (lane 4). 4 µL Page-Ruler Prestained Protein Ladder (Product # 26619) per well was loaded onto a 14% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/PBST for at least 1 hour at room temperature. Histone H3K9me2 antibody (Pro

Rabbit Poly, 100 ug
Hu
ChIP, DB, FACS, ICC, IF, WB

ChIP,

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Dot Blot image

DB,

FACS, ICC, Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies MA5-24678

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies. Western blot analysis was performed on HeLa (Lane 1), HCT 116 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4) and JURKAT (Lane 5). The blot was probed with Di-Methyl-Histone H3 (Lys18) (Product # MA5-24678, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Methyl-Histone H3 (Lys18) was observed across the cell lines tested.

Rabbit Mono [RM171], 100 ug
Hu
AP, ChIP, DB, ELISA, IF, WB

AP,

ChIP,

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Dot Blot image

DB, ELISA, Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies MA5-24677

Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies. Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), HCT 116 (Lane 2), MDA-MB-231 (Lane 3), Jurkat (Lane 4) and MCF7 (Lane 5). The blot was probed with Di-Methyl-Histone H3 (Lys18) (Product # MA5-24677, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Methyl-Histone H3 (Lys18) was obse

Rabbit Mono [RM168], 100 ug
Hu
AP, DB, ELISA, IF, WB

AP,

Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies, Dot Blot image

DB, ELISA, Di-Methyl-Histone H3 antibody, MA5-24677, Invitrogen Antibodies, Immunofluorescence image

Enzyme Linked Immunosorbent Assay

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K9me2 antibody (Product # 49-1007), crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1013 Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me2 antibody (Product # 49-1013). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me2 antibody (Product # 49-1013). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021 Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Cat. no. 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Cat. no. 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019 Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Cat. no. 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4,500.

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Cat. no. 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4,500.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004 Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me2 antibody (Product # 49-1004). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,600.

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me2 antibody (Product # 49-1004). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,600.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Chromatin Immunoprecipitation

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. Enrichment of endogenous Di-Methyl-Histone H3 (Lys9) protein at specific gene loci using Anti-Di-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Di-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (Product # 49-1007, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

DB,

ELISA,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1013

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, anti-H3K27me2 antibody (Product # 49-1013), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.5 million cells and various amounts of anti-H3K27me2 antibody (2, 5, and 10 µg). IgG (5 µg&frasl;IP) was used as negative IP control. qPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. This figure shows the recovery, expressed as a percentage of inp

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

DB,

ELISA,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3R17me2 crude serum (Product # 49-1021), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3R17me2 crude serum (2, 5, 10, and 15 µl). IgG (5 µg&frasl;IP) was used as negative IP control. This figure shows the recovery, expressed as a percentage of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR ana

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3K79me2 crude serum (Product # 49-1019), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3K79me2 crude serum (2, 7, and 15 µl). Additionally, ChIP was performed with 2 and 15 µl of crude serum after incubation with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 µg&frasl;IP) was used as negative IP control. qPCR

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3K4me2 crude serum (Product # 49-1004) and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3K4me2 crude serum (2, 5, 10, or 15 µl). IgG (5 µg&frasl;IP) was used as negative IP control. qPCR was performed with primers for the promoter of the fructose-bisphosphate aldolase A (ALDOA) gene and for the coding region of the myogenic differenti

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 720092

Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies. Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone H3K9me2 Rabbit Polyclonal Antibody (Product # 720092, 3 ug) on sheared chromatin from 2 million HeLa cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the inactive SAT2 satellite repeat, used as positive control target gene, and promoters of the active

Rabbit Poly, 100 ug
Hu
ChIP, DB, FACS, ICC, IF, WB

DB,

FACS, ICC, Di-Methyl-Histone H3 antibody, 720092, Invitrogen Antibodies, Immunofluorescence image

IF,

Supplier data
Invitrogen Antibodies MA5-24678

Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies. Enrichment of endogenous Di-Methyl-Histone H3 (Lys23) protein at specific gene loci using Anti- Di-Methyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Di-Methyl-Histone H3 (Lys23) Rabbit Monoclonal Antibody (Product # MA5-24678, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR wi

Rabbit Mono [RM171], 100 ug
Hu
AP, ChIP, DB, ELISA, IF, WB

AP,

DB, ELISA, Di-Methyl-Histone H3 antibody, MA5-24678, Invitrogen Antibodies, Immunofluorescence image

IF,

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

2 matching Di-Methyl-Histone H3 antibodies for Dot Blot from 7 suppliers

Superstar Kits

1 Applications, 7 Species currently selected

2 matching Di-Methyl-Histone H3 antibodies for Dot Blot after applying filters

Dot Blot - Use Filter to switch application

Western Blot

Enzyme Linked Immunosorbent Assay

Chromatin Immunoprecipitation

Immunofluorescence

Flow Cytometry

Peptide ELISA

All None
	WB	ChIP	IF	ELISA	ICC	Pep-ELISA	FACS	DB	IHC-P	IP	AP	IHC-F
Human	48	24	23	21	15	11	11	10	8	6	5	1
Mouse	21	3	5	10	5	6	4		8	2		1
Rat	20	3	5	10	5	6	4		8	2		1
Chicken	9	1		8					1			1
Yeast	3			2
Drosophila	1
Bovine	1	1		1					1			1