Histone H2B Antibodies for Affinity purification

22 matching Histone H2B antibodies for Affinity purification after applying filters

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View only Histone H2B Monoclonal Antibodies or Histone H2B Polyclonal Antibodies

Western Blot - Use Filter to switch application

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated with sodium butyrate (5 nM for 24 hr) (Lane 2), COS-7 (Lane 3), and COS-7 treated with sodium butyrate (5 nM for 24 hr) (Lane 4). The blot was probed with Anti-Acetyl-Histone H2B (Lys12) polyclonal antibody (Product # PA5-40100, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 &micr

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Immunocytochemistry image

ICC,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies PA5-40103

Histone H2B antibody, PA5-40103, Invitrogen Antibodies. Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the anti-H2BK15ac antibody (Product # PA5-40103). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, IF, WB

AP,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies MA5-23518

Histone H2B antibody, MA5-23518, Invitrogen Antibodies. HeLa cells were treated with colcemid to block the cell cycle in metaphase and 15 µg of histone extracts of these cells were analyzed by Western Blot using a Phospho-Histone H2B pSer14 monoclonal antibody (Product # MA5-23518) at a dilution of 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Mouse Mono [MA5-23518], 50 ug
Hu
AP, ELISA, WB

AP,

ELISA

Enzyme Linked Immunosorbent Assay

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H2BK12ac antibody (Product # PA5-40100) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:38,200.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

ChIP,

ICC,

IF,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies PA5-40103

Histone H2B antibody, PA5-40103, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H2BK15ac antibody (Product # PA5-40103) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:29,700.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, IF, WB

AP,

ChIP,

IF,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23518 Histone H2B antibody, MA5-23518, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H2BS14p antibody (Product # MA5-23518).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.

Histone H2B antibody, MA5-23518, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H2BS14p antibody (Product # MA5-23518).The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.

Mouse Mono [MA5-23518], 50 ug
Hu
AP, ELISA, WB

AP,

Histone H2B antibody, MA5-23518, Invitrogen Antibodies, Western Blot image

Immunofluorescence

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. HeLa cells were stained with the anti-H2BK12ac antibody (Product # PA5-40100) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK12ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

ChIP,

ELISA,

ICC,

Supplier data
Invitrogen Antibodies PA5-40103

Histone H2B antibody, PA5-40103, Invitrogen Antibodies. HeLa cells were stained with the anti-H2BK15ac antibody (Product # PA5-40103) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H2BK15ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, IF, WB

AP,

ChIP,

ELISA,

Immunocytochemistry

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. Immunofluorescence analysis of Acetyl-Histone H2B (Lys12) was performed using 70% confluent log phase HeLa cells treated with sodium butyrate. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H2B (Lys12) Rabbit Polyclonal Antibody (Product # PA5-40100) at 1:100 dilution in 0.1% BSA, incubated overnight at 4 degree Celsius and

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

ChIP,

ELISA,

IF,

Chromatin Immunoprecipitation

Histone H2B antibodies are often published with	Associated citations
Histone H3	10.1%
Histone Cluster 3 H3	10.1%
Erk1	6.1%
GAPDH	5.1%
Histone Cluster 1 H2A Family Member M	5.1%
c-Myc	4.0%
BAX	4.0%
Synaptotagmin 1	4.0%
p65	4.0%
Histone Cluster 1 H2B Family Member O	3.0%

Histone H2B antibodies are often published with

Associated citations

10.1%

10.1%

6.1%

5.1%

Histone Cluster 1 H2A Family Member M

5.1%

4.0%

4.0%

4.0%

4.0%

Histone Cluster 1 H2B Family Member O

3.0%

All None
	WB	IHC-P	ELISA	IF	IHC-F	ICC	ChIP	FACS	IP	AP	DB
Human	109	58	52	47	44	29	9	5	5	3	3
Mouse	88	55	39	37	43	21	1	2	1
Rat	49	22	12	8	16	9	1	3	2
Bovine	17	16	2	10	13	10	2	1	1
Primate	17	12	1	10	11	9	1	2
Canine	14	14	2	8	9	8		1
Chicken	14	14	1	5	11	5	1
Zebrafish	10	7		3	6	3	1
Drosophila	5	4			3
Rabbit	3	3	2		1
Hamster	3	1		1		1	1	2	1
Mammals	2	2			1
Yeast	2		1				2		2
Bacteria	1	1			1
Xenopus	1
Vertebrates	1		1	1		1
Porcine	1	1	1
Fish	1		1			1
Equine	1	1	1
Feline	1	1		1		1		1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

22 matching Histone H2B antibodies for Affinity purification from 17 suppliers

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Histone H2B information

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1 Applications, 20 Species currently selected

22 matching Histone H2B antibodies for Affinity purification after applying filters

Western Blot - Use Filter to switch application

Enzyme Linked Immunosorbent Assay

Immunofluorescence

Immunocytochemistry

Chromatin Immunoprecipitation

Related antibodies