SMAD2 Antibodies for Immunoprecipitation

37 matching SMAD2 antibodies for Immunoprecipitation after applying filters

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Supplier data
Invitrogen Antibodies 700050

SMAD2 antibody, 700050, Invitrogen Antibodies. Immunoprecipitation of SMAD2 pThr8 was performed on K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 µg of SMAD2 pThr8 monoclonal antibody (Product # 700050) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti- rabbit coated Dynabeads (Product # 11204D), washed extensively, and eluted with NuPAGE™ LDS Sample Buffer (Product # NP0007). Samples were resolved onto NuPAGE™ 4-12% Bis-Tr

Rabbit Mono [700050], 100 ug
Hu
ChIP, FACS, IF, IP, WB

SMAD2 antibody, 700050, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

SMAD2 antibody, 700050, Invitrogen Antibodies, Flow Cytometry image

FACS,

SMAD2 antibody, 700050, Invitrogen Antibodies, Immunofluorescence image

IF,

SMAD2 antibody, 700050, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies PA5-29237 SMAD2 antibody, PA5-29237, Invitrogen Antibodies. PA5-29237 immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 µg of SMAD2antibody (Product # PA5-29237) on 10% SDS-PAGE.

SMAD2 antibody, PA5-29237, Invitrogen Antibodies. PA5-29237 immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 µg of SMAD2antibody (Product # PA5-29237) on 10% SDS-PAGE.

Rabbit Poly, 100 ul
Hu
ChIP, ICC, IF, IP, WB

SMAD2 antibody, PA5-29237, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP, ICC, SMAD2 antibody, PA5-29237, Invitrogen Antibodies, Immunofluorescence image

IF,

SMAD2 antibody, PA5-29237, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 701582

SMAD2 antibody, 701582, Invitrogen Antibodies. Immunoprecipitation of phospho SMAD2 was performed on K562 cells. Antigen-antibody complexes were formed by incubating approximately 500 µg whole cell lysate with 5 µg of SMAD2 pSer465/467 monoclonal antibody (Product # 701582) rotating 60 min at RT. The immune complexes were captured on 625 µg of anti- rabbit coated Dynabeads (Product # 11204D) and washed extensively. They were then eluted and analyzed using the Jess Simple Western system. Lane 1 is input, lane 2 IP without an

Rabbit Mono [8H1L19], 100 ug
Hu
ICC, IF, IP, WB

ICC,

SMAD2 antibody, 701582, Invitrogen Antibodies, Immunofluorescence image

IF,

SMAD2 antibody, 701582, Invitrogen Antibodies, Western Blot image

Supplier data
Proteintech Group 12570-1-AP SMAD2 antibody, 12570-1-AP, Proteintech Group.

Rabbit Poly, 150 ul
Hu, Mo, Rt
ELISA, IF, IHC-F, IHC-P, IP, WB

ELISA,

SMAD2 antibody, 12570-1-AP, Proteintech Group, Immunofluorescence image

IF,

SMAD2 antibody, 12570-1-AP, Proteintech Group, Immunohistochemistry frozen image

IHC-F,

SMAD2 antibody, 12570-1-AP, Proteintech Group, Immunohistochemistry paraffin image

IHC-P,

SMAD2 antibody, 12570-1-AP, Proteintech Group, Western Blot image

Supplier data
Cell Signaling Technology 18338T SMAD2 antibody, 18338T, Cell Signaling Technology.

Rabbit Mono [18338T], 20 ul
Ha, Hu, Rt
AP, ChIP, FACS, ICC, IF, IP, WB

AP,

SMAD2 antibody, 18338T, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

SMAD2 antibody, 18338T, Cell Signaling Technology, Flow Cytometry image

FACS,

SMAD2 antibody, 18338T, Cell Signaling Technology, Immunocytochemistry image

ICC,

SMAD2 antibody, 18338T, Cell Signaling Technology, Immunofluorescence image

IF,

SMAD2 antibody, 18338T, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 18338S SMAD2 antibody, 18338S, Cell Signaling Technology.

Rabbit Mono [18338S], 100 ul
Ha, Hu, Rt
ChIP, FACS, ICC, IF, IP, WB

SMAD2 antibody, 18338S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

SMAD2 antibody, 18338S, Cell Signaling Technology, Flow Cytometry image

FACS,

SMAD2 antibody, 18338S, Cell Signaling Technology, Immunocytochemistry image

ICC,

SMAD2 antibody, 18338S, Cell Signaling Technology, Immunofluorescence image

IF,

SMAD2 antibody, 18338S, Cell Signaling Technology, Western Blot image

Supplier data
GeneTex GTX111131

SMAD2 antibody, GTX111131, GeneTex. SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 μg of SMAD2antibody (GTX111131) 10% SDS-PAGE The immunoprecipitated SMAD2protein was detected by SMAD2antibody (GTX111131) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Rabbit Poly, 100 ul
Hu, Mo
ChIP, ICC, IF, IP, WB

SMAD2 antibody, GTX111131, GeneTex, Chromatin Immunoprecipitation image

ChIP,

SMAD2 antibody, GTX111131, GeneTex, Immunocytochemistry image

ICC,

SMAD2 antibody, GTX111131, GeneTex, Immunofluorescence image

IF,

Western Blot

Supplier data
Boster Biological Technology P00090

Rabbit Mono [P00090], 100 ug
Hu, Mo, Rt
IHC-F, IHC-P, IP, WB

IHC-F, IHC-P, IP

Supplier data
Boster Biological Technology M00090 SMAD2 antibody, M00090, Boster Biological Technology. Western blot analysis of Smad2 expression in Jurkat cell lysate (M00090). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis

SMAD2 antibody, M00090, Boster Biological Technology. Western blot analysis of Smad2 expression in Jurkat cell lysate (M00090). <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis

Rabbit Mono [M00090], 100 ug
Hu, Rt
FACS, ICC, IF, IHC-F, IHC-P, IP, WB

FACS, ICC, IF, IHC-F, IHC-P, IP

Supplier data
Boster Biological Technology A00090 SMAD2 antibody, A00090, Boster Biological Technology. Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090).<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>After Electrophoresis

SMAD2 antibody, A00090, Boster Biological Technology. Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090).<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>After Electrophoresis

Rabbit Poly, 100 ug
Hu, Mo, Rt
IP, WB

Supplier data
Novus Biologicals NBP2-67376 SMAD2 antibody, NBP2-67376, Novus Biologicals. Western Blot: Smad2 Antibody (SP06-05) [NBP2-67376] - Analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: PC12

SMAD2 antibody, NBP2-67376, Novus Biologicals. Western Blot: Smad2 Antibody (SP06-05) [NBP2-67376] - Analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: PC12

Rabbit Mono [SP06-05], 100 ul
Hu, Rt
FACS, ICC, IF, IHC-F, IHC-P, IP, WB

SMAD2 antibody, NBP2-67376, Novus Biologicals, Flow Cytometry image

FACS,

SMAD2 antibody, NBP2-67376, Novus Biologicals, Immunocytochemistry image

ICC,

SMAD2 antibody, NBP2-67376, Novus Biologicals, Immunofluorescence image

IF, IHC-F, SMAD2 antibody, NBP2-67376, Novus Biologicals, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
ABclonal Technology A0440 SMAD2 antibody, A0440, ABclonal Technology. Western blot analysis of extracts of HeLa cell line, using SMAD2 antibody.

SMAD2 antibody, A0440, ABclonal Technology. Western blot analysis of extracts of HeLa cell line, using SMAD2 antibody.

Rabbit Poly, 200 ul
Bac, Hu, Mo, Rb
IF, IHC-F, IHC-P, IP, WB

IF, IHC-F, IHC-P, IP

Supplier data
Invitrogen Antibodies 700050

SMAD2 antibody, 700050, Invitrogen Antibodies. Western blot analysis of Phospho-SMAD2 pThr8 in serum-starved Hela cells, untreated (lane 1) and treated with TGF-beta (10 ng/mL, 30 min) (lane 2) using a Phospho-SMAD2 pThr8 recombinant rabbit monoclonal antibody (Product # 700050) at a dilution of 1 µg/mL. Lanes 3-5 represent competition experiments on lysate from HeLa cells treated with TGF-b: no preincubation with peptide (lane 3), preinicubation with the nonphospho peptide (lane 4) and preincubation with the phospho-peptide (lane 5),

Rabbit Mono [700050], 100 ug
Hu
ChIP, FACS, IF, IP, WB

ChIP,

FACS,

IF,

SMAD2 antibody, 700050, Invitrogen Antibodies, Immunoprecipitation image

Supplier data
Invitrogen Antibodies PA5-29237

SMAD2 antibody, PA5-29237, Invitrogen Antibodies. Knockdown of SMAD2 was achieved by transfecting HeLa with SMAD2 specific siRNAs (Silencer® select Product # s8397, s8399). Western blot analysis (Fig. a) was performed using whole cell extracts from the SMAD2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with SMAD2 Polyclonal Antibody (Product # PA5-29237, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conju

Rabbit Poly, 100 ul
Hu
ChIP, ICC, IF, IP, WB

ChIP, ICC, SMAD2 antibody, PA5-29237, Invitrogen Antibodies, Immunofluorescence image

IF,

SMAD2 antibody, PA5-29237, Invitrogen Antibodies, Immunoprecipitation image

Supplier data
Invitrogen Antibodies 701582 SMAD2 antibody, 701582, Invitrogen Antibodies. Western blot analysis was performed to detect SMAD2 (pSpS465/467) protein by loading 30

SMAD2 antibody, 701582, Invitrogen Antibodies. Western blot analysis was performed to detect SMAD2 (pSpS465/467) protein by loading 30

Rabbit Mono [8H1L19], 100 ug
Hu
ICC, IF, IP, WB

ICC,

IF,

SMAD2 antibody, 701582, Invitrogen Antibodies, Immunoprecipitation image

Supplier data
Invitrogen Antibodies MA5-14996 SMAD2 antibody, MA5-14996, Invitrogen Antibodies. Western blot analysis of Smad2 in extracts from HeLa and H1975 cell lines using Smad2 monoclonal antibody (Product # MA5-14996).

SMAD2 antibody, MA5-14996, Invitrogen Antibodies. Western blot analysis of Smad2 in extracts from HeLa and H1975 cell lines using Smad2 monoclonal antibody (Product # MA5-14996).

Rabbit Mono [R.542.9], 100 ul
Hu, Pr
ICC, IF, IP, WB

ICC,

SMAD2 antibody, MA5-14996, Invitrogen Antibodies, Immunofluorescence image

IF, IP

Supplier data
Invitrogen Antibodies PA5-17621

SMAD2 antibody, PA5-17621, Invitrogen Antibodies. Western blot analysis was performed on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HeLa (Lane 2), C2C12 (Lane 3), HEK 293 (Lane 4), MCF7 (Lane 5), U-87 MG (Lane 6), A-431 (Lane 7) and U-2 OS (Lane 8). The blot was probed with Anti-SMAD2/SMAD3 Polyclonal Antibody (Product # PA5-17621, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 53 kD

Rabbit Poly, 100 ul
Hu, Mo, Pr, Rt
IP, WB

Supplier data
Proteintech Group 12570-1-AP SMAD2 antibody, 12570-1-AP, Proteintech Group.

Rabbit Poly, 150 ul
Hu, Mo, Rt
ELISA, IF, IHC-F, IHC-P, IP, WB

ELISA,

IF,

IHC-F,

IHC-P,

SMAD2 antibody, 12570-1-AP, Proteintech Group, Immunoprecipitation image

Supplier data
Lifespan Biosciences LS-B7850 SMAD2 antibody, LS-B7850, Lifespan Biosciences.

Mouse Mono [LS-B7850], 1 Vial
Hu, Mo, Rt
ELISA, IHC-F, IHC-P, IP, WB

ELISA,

SMAD2 antibody, LS-B7850, Lifespan Biosciences, Immunohistochemistry frozen image

IHC-F,

SMAD2 antibody, LS-B7850, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Lifespan Biosciences LS-C330963 SMAD2 antibody, LS-C330963, Lifespan Biosciences. Western blot analysis of extracts of HeLa cells, using SMAD2 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

SMAD2 antibody, LS-C330963, Lifespan Biosciences. Western blot analysis of extracts of HeLa cells, using SMAD2 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

Rabbit Poly, 1 Vial
Hu, Mo, Rt
IF, IHC-F, IP, WB

IF, IHC-F, IP

Related antibodies

SMAD2 antibodies are often published with	Associated citations
SMAD Family Member 3	8.5%
Erk1	7.9%
Fibronectin 1	7.2%
SMAD Family Member 1	6.6%
Vimentin	5.9%
GAPDH	4.9%
Cadherin 1	4.9%
p38	4.6%
Ring Finger Protein 19A, RBR E3 Ubiquitin Protein Ligase	3.9%
DNA Polymerase Delta Interacting Protein 2	3.6%

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

37 matching SMAD2 antibodies for Immunoprecipitation from 26 suppliers

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SMAD2 information

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1 Applications, 19 Species currently selected

37 matching SMAD2 antibodies for Immunoprecipitation after applying filters

Immunoprecipitation - Use Filter to switch application

Western Blot

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	WB	IHC-P	IF	IHC-F	ELISA	ICC	FACS	IP	ChIP	DB	AP	Pep-ELISA	FA
Human	342	143	130	111	102	81	46	24	15	6	3	2	1
Rat	246	117	80	96	65	54	24	17	8		1	1	1
Mouse	239	96	68	75	75	41	24	15	10		1	1	1
Primate	32	15	11	12	7	10	8	7	4				1
Bovine	30	22	8	15	13	8	4					2
Hamster	25	19	20	19		20	6	2	2		1
Chicken	23	17	6	13	8	8	1
Zebrafish	19	9	7	7	4	7	2
Rabbit	16	10	6	9	5	6	1	1
Canine	15	14	1	6	9	1	1					1
Guinea Pig	13	10	4	6	5	5	1					1
Sheep	8	6	4	5	3	5	1
Equine	5	5		1	4	1
Bacteria	4	1	1	1		1		1
Xenopus	4	1		1	1	1
Bat	2	1		1	1	1
Drosophila	1	1		1
Frog	1	1		1
Feline	1