Histone H3 Antibodies for Peptide ELISA

67 matching Histone H3 antibodies for Peptide ELISA after applying filters

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Supplier data
Invitrogen Antibodies 49-1012

Histone H3 antibody, 49-1012, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K27me1 crude serum, the anti-H3K4me3 antibody (Product # 49-1012), and the column flow through obtained from the antibody purification step. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:760.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1016

Histone H3 antibody, 49-1016, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K36me1 crude serum, the anti-H3K36me1 antibody (Product # 49-1016), and the column flow through obtained from the antibody purification step. The antigen used was the peptide including the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:1,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1016, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1016, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1016, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA, ICC, Histone H3 antibody, 49-1016, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1016, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1011 Histone H3 antibody, 49-1011, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9asS10p crude serum (Product # 49-1011). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:89,000.

Histone H3 antibody, 49-1011, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9asS10p crude serum (Product # 49-1011). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:89,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1003 Histone H3 antibody, 49-1003, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me1 crude serum (Product # 49-1003). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:3, 800.

Histone H3 antibody, 49-1003, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me1 crude serum (Product # 49-1003). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:3, 800.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1006 Histone H3 antibody, 49-1006, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9me1 crude serum (Product # 49-1006). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:50,000.

Histone H3 antibody, 49-1006, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9me1 crude serum (Product # 49-1006). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:50,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1008

Histone H3 antibody, 49-1008, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K9me3 crude serum, the anti-H3K9me3 polyclonal antibody (Product # 49-1008), and the column flow through obtained from the antibody purification step. The antigen used to coat the ELISA plate was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:13, 500.

Rabbit Poly, 50 ug
Hu, Mo
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1005

Histone H3 antibody, 49-1005, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K4me3 crude serum, the anti-H3K4me3 antibody (Product # 49-1005), and the column flow through obtained from the antibody purification step. The antigen used was a peptide containing the histone H3 modification (trimethylated lysine 4). By plotting the absorbance against the antibody dilution, the titer of the affinity-purified antibody was estimated to be 1:50,000.

Rabbit Poly, 24 ug
Hu, Mo
ChIP, ELISA, IF, Pep-ELISA, WB

Histone H3 antibody, 49-1005, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1005, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1005, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1005, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23514

Histone H3 antibody, MA5-23514, Invitrogen Antibodies. To test the specificity, an ELISA was performed using a serial dilution of Methyl-Histone H3 (Lys4) monoclonal antibody (Product # MA5-23514). The wells were coated with peptides containing the unmodified H3K4 as well as the mono-, di- and trimethylated H3K4 and the monomethylated H3K9. Figure 2 shows a high specificity of the antibody for the modification of interest.

Mouse Mono [MA5-23514], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA, ICC, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1018 Histone H3 antibody, 49-1018, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me1 crude serum in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the anti-H3K27me3 crude serum was estimated to be 1:30,000.

Histone H3 antibody, 49-1018, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me1 crude serum in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the anti-H3K27me3 crude serum was estimated to be 1:30,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Histone H3 antibody, 49-1018, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1018, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1018, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1018, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1024

Histone H3 antibody, 49-1024, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H4K20me3 crude serum, purified anti-H4K20me3 antibodies, and the column flow through obtained from the antibody purification step. The antigen used was the peptide including the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:700 for crude serum (Product # 49-1021) and 1:350 for affinity purified antibody.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Histone H3 antibody, 49-1024, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1024, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1024, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1024, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1022 Histone H3 antibody, 49-1022, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H4K8ac crude serum (Product # 49-1022). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:17, 500.

Histone H3 antibody, 49-1022, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H4K8ac crude serum (Product # 49-1022). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:17, 500.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Histone H3 antibody, 49-1022, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, 49-1022, Invitrogen Antibodies, Dot Blot image

DB,

Histone H3 antibody, 49-1022, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H3 antibody, 49-1022, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23516

Histone H3 antibody, MA5-23516, Invitrogen Antibodies. To test the specificity, an ELISA was performed using a serial dilution of Acetyl-Histone H3 (Lys27) monoclonal antibody (Product # MA5-23516). The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure 1 shows a high specificity of the antibody for the peptide containing the modification of interest.

Mouse Mono [MA5-23516], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

Histone H3 antibody, MA5-23516, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Histone H3 antibody, MA5-23516, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA, ICC, Histone H3 antibody, MA5-23516, Invitrogen Antibodies, Immunofluorescence image

IF, WB

Western Blot

Supplier data
Invitrogen Antibodies 49-1012 Histone H3 antibody, 49-1012, Invitrogen Antibodies. Histone extracts (15 μg) from HeLa cells were analysed by Western blot using the anti-H3K27me1 antibody (Product # 49-1012) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Histone H3 antibody, 49-1012, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1016 Histone H3 antibody, 49-1016, Invitrogen Antibodies. Histone extracts from HeLa cells (15 µg) were analyzed by western blot using the anti-H3K36me1 antibody (Product # 49-1016), diluted 1:500 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Histone H3 antibody, 49-1016, Invitrogen Antibodies. Histone extracts from HeLa cells (15 µg) were analyzed by western blot using the anti-H3K36me1 antibody (Product # 49-1016), diluted 1:500 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA, ICC, Histone H3 antibody, 49-1016, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, 49-1016, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1011 Histone H3 antibody, 49-1011, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K9acS10p crude serum (Product # 49-1011), diluted 1:250 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Histone H3 antibody, 49-1011, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K9acS10p crude serum (Product # 49-1011), diluted 1:250 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Histone H3 antibody, 49-1011, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1003

Histone H3 antibody, 49-1003, Invitrogen Antibodies. Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with TrichostatinA (0.4µg/ml for 16h) (Lane 2), HeLa (Lane 3), Hela treated with TrichostatinA (0.4µg/ml for 16h) (Lane 4), NIH3T3 (Lane 5), NIH3T3 treated with TrichostatinA (0.4µg/ml for 16h) (Lane 6), COLO 205 (Lane 7) and COLO 205 treated with TrichostatinA (0.4µg/ml for 16h) (Lane 8). The blot was probed with Anti-Methyl-Histone H3 (L

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Histone H3 antibody, 49-1003, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1006 Histone H3 antibody, 49-1006, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K9me1 crude serum (Product # 49-1006), diluted 1:1000 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Histone H3 antibody, 49-1006, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K9me1 crude serum (Product # 49-1006), diluted 1:1000 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Histone H3 antibody, 49-1006, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1008

Histone H3 antibody, 49-1008, Invitrogen Antibodies. Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the anti-H3K9me3 antibody (Product # 49-1008). The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu, Mo
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Histone H3 antibody, 49-1008, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1005

Histone H3 antibody, 49-1005, Invitrogen Antibodies. Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), NIH/3T3 (Lane 2), A-431 (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and C2C12 (Lane 6). The blot was probed with Anti-Tri-Methyl-Histone H3 (Lys4) (Product # 49-1005, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Tri-Methyl-Histone H3

Rabbit Poly, 24 ug
Hu, Mo
ChIP, ELISA, IF, Pep-ELISA, WB

ChIP,

ELISA,

IF,

Histone H3 antibody, 49-1005, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies MA5-23514 Histone H3 antibody, MA5-23514, Invitrogen Antibodies. Histone extracts (15 µg) from HeLa cells were analyzed by Western Blot using a Methyl-Histone H3 (Lys4) monoclonal antibody (Product # MA5-23514) at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Histone H3 antibody, MA5-23514, Invitrogen Antibodies. Histone extracts (15 µg) from HeLa cells were analyzed by Western Blot using a Methyl-Histone H3 (Lys4) monoclonal antibody (Product # MA5-23514) at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Mouse Mono [MA5-23514], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

ELISA, ICC, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1018 Histone H3 antibody, 49-1018, Invitrogen Antibodies. Histone extracts of HeLa cells were analyzed by western blot using the anti-H3K79me1 crude serum (Product # 49-1018), diluted 1:1,000 and 1:2,000 in TBS-Tween containing 5% milk. The molecular weight marker is shown on the left. The location of the protein of interest is indicated with an arrow.

Histone H3 antibody, 49-1018, Invitrogen Antibodies. Histone extracts of HeLa cells were analyzed by western blot using the anti-H3K79me1 crude serum (Product # 49-1018), diluted 1:1,000 and 1:2,000 in TBS-Tween containing 5% milk. The molecular weight marker is shown on the left. The location of the protein of interest is indicated with an arrow.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1024 Histone H3 antibody, 49-1024, Invitrogen Antibodies. Histone (acid) extracts of NB4 cells were analysed by Western blot using the anti-H4K20me3 antibody (Product # 49-1024), diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.

Histone H3 antibody, 49-1024, Invitrogen Antibodies. Histone (acid) extracts of NB4 cells were analysed by Western blot using the anti-H4K20me3 antibody (Product # 49-1024), diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1022

Histone H3 antibody, 49-1022, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H4K8ac crude serum (Product # 49-1022), diluted 1:250 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right. Lane 2 shows the result of the western analysis with the crude serum; lane 1 shows the same analysis after incubation of the crude serum with 750 pmol blocking peptide for 1 hour at room temperature.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Immunofluorescence

Supplier data
Invitrogen Antibodies 49-1012

Histone H3 antibody, 49-1012, Invitrogen Antibodies. Human osteosarcoma (U2OS) cells were stained with the anti-H3K27me1 antibody (Product # 49-1012) and with DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K27me1 antibody (left) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. Figure B, C, D and E: staining of the cells

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1012, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1016

Histone H3 antibody, 49-1016, Invitrogen Antibodies. Human osteosarcoma (U2OS) cells were stained with the anti-H3K36me1 antibody (Product # 49-1016) and with DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. Figure A: cells were immunofluorescently labeled with the H3K36me1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. Figure B, C, D and E: staining of the cells w

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA, ICC, Histone H3 antibody, 49-1016, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA, Histone H3 antibody, 49-1016, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1011 Histone H3 antibody, 49-1011, Invitrogen Antibodies. Immunofluorescence using the anti-H3K9acS10p antibody (Product # 49-1011).

Histone H3 antibody, 49-1011, Invitrogen Antibodies. Immunofluorescence using the anti-H3K9acS10p antibody (Product # 49-1011).

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1011, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1003

Histone H3 antibody, 49-1003, Invitrogen Antibodies. Immunofluorescence analysis of Methyl-Histone H3 (Lys4) was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Methyl-Histone H3 (Lys4) Polyclonal Antibody (Product # 49-1003) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1003, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1006

Histone H3 antibody, 49-1006, Invitrogen Antibodies. HeLa cells were formaldehyde fixed, permeabilized with Triton® X-100 (Dow Chemical Co.), and blocked with PBS containing 2.5% BSA. (A) Cells were labeled with the anti-H3K9me1 crude serum (diluted 1:200 and incubated for 1 hour at room temperature) followed by a dye-labeled goat anti-rabbit secondary antibody. (B) Nuclei were stained using the DNA-specific stain DAPI. Both the anti-H3K9me1 serum sample and DAPI produced clear nuclear staining.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1006, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1008

Histone H3 antibody, 49-1008, Invitrogen Antibodies. HeLa cells were stained with the anti-H3K9me3 antibody (Product # 49-1008) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9me3 antibody (middle) diluted 1:1,000 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The left panel shows staining of the nuclei with DAPI. A merge of both stainings is shown on the right.

Rabbit Poly, 50 ug
Hu, Mo
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1008, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1005

Histone H3 antibody, 49-1005, Invitrogen Antibodies. HeLa cells were stained with the anti-H3K4me3 antibody (Product # 49-1005) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K4me3 antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Rabbit Poly, 24 ug
Hu, Mo
ChIP, ELISA, IF, Pep-ELISA, WB

ChIP,

ELISA,

Pep-ELISA, Histone H3 antibody, 49-1005, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23514

Histone H3 antibody, MA5-23514, Invitrogen Antibodies. Immunofluorescent detection of Methyl-Histone H3 (Lys4) in HeLa cells stained with Methyl-Histone H3 (Lys4) monoclonal antibody (Product # MA5-23514). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K4me1 antibody (left) at a dilution of 1:500 in blocking solution followed by an anti-mouse antibody conjugated to AlexaFluor 594. The middle panel shows staining of the

Mouse Mono [MA5-23514], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

ELISA, ICC, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23516

Histone H3 antibody, MA5-23516, Invitrogen Antibodies. Immunofluorescent detection of Acetyl-Histone H3 (Lys27) in HeLa cells stained with Acetyl-Histone H3 (Lys27) monoclonal antibody (Product # MA5-23516). Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) at a dilution of 1:500 in blocking solution followed by an anti-mouse antibody conjugated to AlexaFluor 594. The middle panel shows staining of th

Mouse Mono [MA5-23516], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

ELISA, ICC, Histone H3 antibody, MA5-23516, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA, WB

Enzyme Linked Immunosorbent Assay

Supplier data
Invitrogen Antibodies 49-1012 Histone H3 antibody, 49-1012, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me1 antibody (Product # 49-1012). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:32,900.

Histone H3 antibody, 49-1012, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me1 antibody (Product # 49-1012). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:32,900.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Histone H3 antibody, 49-1012, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1016

Histone H3 antibody, 49-1016, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K36me1 antibody (Product # 49-1016), crude serum and Flow Through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, Pep-ELISA, WB

ChIP,

DB, ICC,

IF,

Pep-ELISA, Histone H3 antibody, 49-1016, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1011 Histone H3 antibody, 49-1011, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9asS10p crude serum (Cat. no. 49-1011). The antigen used was a peptide containing the histone modifications of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:89,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Histone H3 antibody, 49-1011, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1003 Histone H3 antibody, 49-1003, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me1 antibody (Product # 49-1003). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,800.

Histone H3 antibody, 49-1003, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me1 antibody (Product # 49-1003). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,800.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Histone H3 antibody, 49-1003, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1006 Histone H3 antibody, 49-1006, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9me1 antibody (Product # 49-1006). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.

Histone H3 antibody, 49-1006, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K9me1 antibody (Product # 49-1006). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Histone H3 antibody, 49-1006, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1008

Histone H3 antibody, 49-1008, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human H3K9me3 antibody (Product # 49-1008) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.

Rabbit Poly, 50 ug
Hu, Mo
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Histone H3 antibody, 49-1008, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1005 Histone H3 antibody, 49-1005, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K4me3 antibody (Product # 49-1005). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:18,350.

Histone H3 antibody, 49-1005, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K4me3 antibody (Product # 49-1005). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:18,350.

Rabbit Poly, 24 ug
Hu, Mo
ChIP, ELISA, IF, Pep-ELISA, WB

ChIP,

IF,

Pep-ELISA, Histone H3 antibody, 49-1005, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23514

Mouse Mono [MA5-23514], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

ChIP, ICC, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Immunofluorescence image

IF,

Pep-ELISA, Histone H3 antibody, MA5-23514, Invitrogen Antibodies, Western Blot image

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Histone H3 antibody, 49-1018, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1024 Histone H3 antibody, 49-1024, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H4K20me3 antibody (Product # 49-1024). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.

Histone H3 antibody, 49-1024, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H4K20me3 antibody (Product # 49-1024). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Histone H3 antibody, 49-1024, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1022 Histone H3 antibody, 49-1022, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-human H4K8ac antibody (Product # 49-1022). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:17,500.

Histone H3 antibody, 49-1022, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-human H4K8ac antibody (Product # 49-1022). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:17,500.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Histone H3 antibody, 49-1022, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies MA5-23516

Mouse Mono [MA5-23516], 50 ug
Hu
ChIP, ELISA, ICC, IF, Pep-ELISA, WB

ChIP, ICC, Histone H3 antibody, MA5-23516, Invitrogen Antibodies, Immunofluorescence image

IF,

Pep-ELISA, WB

Chromatin Immunoprecipitation

Histone H3 antibodies are often published with	Associated citations
Histone Cluster 3 H3	19.5%
iNOS	17.2%
p65	4.1%
GFAP	2.5%
GAPDH	2.5%
Coproporphyrinogen Oxidase	2.1%
c-Myc	2.1%
BrdU	2.0%
Erk1	1.9%
BCL2	1.8%

Histone H3 antibodies are often published with

Associated citations

19.5%

17.2%

4.1%

2.5%

2.5%

Coproporphyrinogen Oxidase

2.1%

2.1%

2.0%

1.9%

1.8%

All None
	WB	IF	ELISA	ICC	IHC-P	IHC-F	ChIP	IP	DB	FACS	AP	Pep-ELISA	FA	Bl
Human	3221	1896	1378	1300	1300	890	741	474	400	253	141	79	4	1
Rat	2054	1132	639	747	849	512	343	367	123	157	83	63	2	1
Mouse	2053	1226	675	825	826	500	490	353	288	132	68	65	3	1
Hamster	350	136	46	87	63	48	90	95	67	66	19
Primate	307	221	92	134	96	40	149	148	70	102	21		3
Zebrafish	254	236	53	177	77	34	119	96	77	75	14		2
Bovine	207	113	70	66	72	39	81	90	64	42	11		1
Xenopus	182	114	47	64	38	16	101	100	69	61	15		1
Drosophila	179	101	63	58	49	25	94	87	67	41	9			1
Chicken	153	107	30	64	44	36	61	71	64	18	6
Rabbit	141	75	39	24	54	25	54	58	69	7
Yeast	129	88	37	37	27	17	82	77	69	5	1
Guinea Pig	129	76	27	33	35	18	59	55	64	10	2		1
Porcine	109	113	32	71	28	12	60	58	63	10	3
C.Elegans	105	64	33	26	13	11	66	60	71	6	2
Bacteria	95	59	16	8	8	7	60	56	69	5	7
Feline	80	91	10	50	9	1	51	52	63
Mammals	90	56	27	22	11	9	62	52	63
Equine	89	47	21	6	6	2	57	54	63	2
Canine	89	43	20	2	10	3	51	53	63	1
Plant	80	49	14	6	7	6	56	55	66
Eukaryotes	78	42	10	1	2	1	51	52	63
Sheep	76	44	15	3	6	3	51	54	63	1
Fish	74	44	11	3	2	1	52	52	64
Goat	72	43	11	2	3	2	51	53	63	1
Frog	71	42	10	1	2	1	51	52	63
Donkey	71	42	10	1	2	1	51	52	63
Virus	71	42	10	1	2	1	51	52	63
Goldfish	71	42	10	1	2	1	51	52	63
Prokaryotes	71	42	10	1	2	1	51	52	63
Vertebrates	34	17	11	17	6	6	2	10	2		1
Invertebrates	10	11	9	11	8	8	4	1
Fungus	3		3		3	3		3
Squirrel	1				1	1		1
Duck	1				1	1		1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

67 matching Histone H3 antibodies for Peptide ELISA from 37 suppliers

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Histone H3 information

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67 matching Histone H3 antibodies for Peptide ELISA after applying filters

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