Di-Methyl-Histone H3 Antibodies for Peptide ELISA

6 matching Di-Methyl-Histone H3 antibodies for Peptide ELISA after applying filters

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Peptide ELISA - Use Filter to switch application

Supplier data
Invitrogen Antibodies 49-1013

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K27me2 crude serum, the anti-H3K27me2 antibody (Product # 49-1013), and the column flow through obtained from the antibody purification step. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:4, 350.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Dot Blot image

DB,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. To determine the titer, an ELISA was performed using H3K9me2 Polyclonal Antibody, Rabbit crude serum, the H3K9me2 Polyclonal Antibody, Rabbit antibody (Product # 49-1007), and the column flow through obtained from the antibody purification step. The antigen used was the peptide including the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:160,000 and for the affinity-purified antibody (Product # 49-100

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Dot Blot image

DB,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Immunofluorescence image

IF,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019 Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Product # 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4, 500.

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Product # 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4, 500.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Dot Blot image

DB,

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004 Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me2 crude serum (Product # 49-1004). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:2, 600.

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me2 crude serum (Product # 49-1004). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:2, 600.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Dot Blot image

DB,

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021 Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Product # 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Product # 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Dot Blot image

DB,

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Western Blot

Supplier data
Invitrogen Antibodies 49-1013 Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. Histone extracts (10 µg) from NB4 cells were analyzed by western blot using anti-H3K27me2 antibody (Product # 49-1013), diluted 1:500 in TBS-Tween. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. Western blot analysis was performed on HeLa (Lane 1), HCT 116 (Lane 2), MDA-MB-231 (Lane 3), MCF7 (Lane 4) and JURKAT (Lane 5). The blot was probed with Anti- Di-Methyl-Histone H3 (Lys9) (Product # 49-1007, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to Di-Methyl-Histone H3 (Lys9) was observed across the cell lines tested.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

ELISA,

IF,

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Peptide ELISA image

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1019 Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K79me2 crude serum (Product # 49-1019), diluted 1:250 in TBS-Tween containing 5% milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K79me2 crude serum (Product # 49-1019), diluted 1:250 in TBS-Tween containing 5% milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1004 Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K4me2 crude serum (Product # 49-1004), diluted 1:750 in TBS-Tween containing 5% skim milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K4me2 crude serum (Product # 49-1004), diluted 1:750 in TBS-Tween containing 5% skim milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Supplier data
Invitrogen Antibodies 49-1021 Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3R17me2 crude serum (Product # 49-1021), diluted 1:250 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3R17me2 crude serum (Product # 49-1021), diluted 1:250 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated with an arrow; the marker (in kDa) is shown on the right.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

ELISA,

Pep-ELISA

Enzyme Linked Immunosorbent Assay

Supplier data
Invitrogen Antibodies 49-1013 Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me2 antibody (Product # 49-1013). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K27me2 antibody (Product # 49-1013). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the anti-H3K9me2 antibody (Product # 49-1007), crude serum and Flow through. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

ChIP,

DB,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019 Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Cat. no. 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4,500.

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. To determine the titer, an ELISA was performed using anti-H3K79me2 crude serum (Cat. no. 49-1019). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:4,500.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004 Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. To determine the titer, an ELISA was performed using a serial dilution of the anti-H3K4me2 antibody (Product # 49-1004). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,600.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021 Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Cat. no. 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. To determine the titer, an ELISA was performed using the anti-H3R17me2 crude serum (Cat. no. 49-1021). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1:40,000.

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

ChIP,

DB,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Western Blot image

Chromatin Immunoprecipitation

Supplier data
Invitrogen Antibodies 49-1013

Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, anti-H3K27me2 antibody (Product # 49-1013), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.5 million cells and various amounts of anti-H3K27me2 antibody (2, 5, and 10 µg). IgG (5 µg&frasl;IP) was used as negative IP control. qPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. This figure shows the recovery, expressed as a percentage of inp

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

DB,

ELISA,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1013, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1007

Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies. Enrichment of endogenous Di-Methyl-Histone H3 (Lys9) protein at specific gene loci using Anti-Di-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Di-Methyl-Histone H3 (Lys9) Rabbit Polyclonal Antibody (Product # 49-1007, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, Pep-ELISA, WB

DB,

ELISA,

IF,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1007, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1019

Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3K79me2 crude serum (Product # 49-1019), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3K79me2 crude serum (2, 7, and 15 µl). Additionally, ChIP was performed with 2 and 15 µl of crude serum after incubation with 5 nmol blocking peptide for 1 hour at room temperature. IgG (5 µg&frasl;IP) was used as negative IP control. qPCR

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1019, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1004

Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3K4me2 crude serum (Product # 49-1004) and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3K4me2 crude serum (2, 5, 10, or 15 µl). IgG (5 µg&frasl;IP) was used as negative IP control. qPCR was performed with primers for the promoter of the fructose-bisphosphate aldolase A (ALDOA) gene and for the coding region of the myogenic differenti

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1004, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 49-1021

Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies. ChIP assays were performed using human osteosarcoma (U-2OS) cells, the anti-H3R17me2 crude serum (Product # 49-1021), and optimized PCR primer sets for qPCR. Each ChIP assay used sheared chromatin from 1.6 million cells and a volume of anti-H3R17me2 crude serum (2, 5, 10, and 15 µl). IgG (5 µg&frasl;IP) was used as negative IP control. This figure shows the recovery, expressed as a percentage of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR ana

Rabbit Poly, 100 ul
Hu
ChIP, DB, ELISA, Pep-ELISA, WB

DB,

ELISA,

Pep-ELISA, Di-Methyl-Histone H3 antibody, 49-1021, Invitrogen Antibodies, Western Blot image

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

6 matching Di-Methyl-Histone H3 antibodies for Peptide ELISA from 7 suppliers

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6 matching Di-Methyl-Histone H3 antibodies for Peptide ELISA after applying filters

Peptide ELISA - Use Filter to switch application

Western Blot

Enzyme Linked Immunosorbent Assay

Chromatin Immunoprecipitation

Immunofluorescence

Dot Blot

All None
	WB	ChIP	ELISA	IF	ICC	FACS	DB	Pep-ELISA	AP	IP	IHC-P
Human	26	17	17	14	9	8	8	5	4	2	1
Mouse	10	2	9	1	1	1				1	1
Rat	10	2	9	1	1	1				1	1
Chicken	5		5