Histone H2B Antibodies for Chromatin Immunoprecipitation

13 matching Histone H2B antibodies for Chromatin Immunoprecipitation after applying filters

Click each Histone H2B antibody that interests you to view the full Histone H2B antibody datasheet.
View only Histone H2B Monoclonal Antibodies or Histone H2B Polyclonal Antibodies

Chromatin Immunoprecipitation - Use Filter to switch application

Supplier data
Novus Biologicals NBP2-59270

Histone H2B antibody, NBP2-59270, Novus Biologicals. Chromatin Immunoprecipitation: Histone H2B Antibody [NBP2-59270] - ChIP assays were performed using human HeLa cells, the antibody against H2Bpan and optimized PCR primer sets for qPCR. ChIP was performed with the "LowCell# ChIP" kit on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAP

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, IP, WB

DB,

Histone H2B antibody, NBP2-59270, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, NBP2-59270, Novus Biologicals, Immunocytochemistry image

ICC,

Histone H2B antibody, NBP2-59270, Novus Biologicals, Immunofluorescence image

IF,

Histone H2B antibody, NBP2-59270, Novus Biologicals, Immunoprecipitation image

IP,

Histone H2B antibody, NBP2-59270, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-59271

Histone H2B antibody, NBP2-59271, Novus Biologicals. Chromatin Immunoprecipitation: Histone H2B Antibody [NBP2-59271] - ChIP assays were performed using human HeLa cells, the antibody against H2BK12ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promote

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

DB,

Histone H2B antibody, NBP2-59271, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, NBP2-59271, Novus Biologicals, Immunofluorescence image

IF,

Histone H2B antibody, NBP2-59271, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-59272

Histone H2B antibody, NBP2-59272, Novus Biologicals. Chromatin Immunoprecipitation: Histone H2B Antibody [NBP2-59272] - ChIP assays were performed using human HeLa cells, the antibody against H2BK15ac and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 0.5, 1, 2 and, 5 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promote

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

DB,

Histone H2B antibody, NBP2-59272, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, NBP2-59272, Novus Biologicals, Immunofluorescence image

IF,

Histone H2B antibody, NBP2-59272, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-54770 Histone H2B antibody, NBP2-54770, Novus Biologicals. Chromatin Immunoprecipitation (ChIP): Histone H2B Antibody (1B3F12/A9) [NBP2-54770] - ChIP using anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]. Gcn5 regulates H2Bub and H3K9ac at several loci. ChIP-qPCR analysis of H2Bub and H3K9ac, normalized to H2B, at several loci. n=3 each, the wild-type average was set to 1. PMID: 27922451

Histone H2B antibody, NBP2-54770, Novus Biologicals. Chromatin Immunoprecipitation (ChIP): Histone H2B Antibody (1B3F12/A9) [NBP2-54770] - ChIP using anti-Ubiquitinated Histone H2B (Lys123) [1B3F12/A9]. Gcn5 regulates H2Bub and H3K9ac at several loci. ChIP-qPCR analysis of H2Bub and H3K9ac, normalized to H2B, at several loci. n=3 each, the wild-type average was set to 1. PMID: 27922451

Mouse Mono [1B3F12/A9], 100 ul
Hu, Yt
ChIP, ELISA, IP, WB

Histone H2B antibody, NBP2-54770, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, NBP2-54770, Novus Biologicals, Immunoprecipitation image

IP,

Histone H2B antibody, NBP2-54770, Novus Biologicals, Western Blot image

Supplier data
Novus Biologicals NBP2-59149

Histone H2B antibody, NBP2-59149, Novus Biologicals. Chromatin Immunoprecipitation: Histone H2B Antibody (C.15200232) [NBP2-59149] - ChIP results obtained with the antibody against H2BK123ub on sheared chromatin from WT yeast cells, yeast cells with higher steady state levels of H2BK123ub and yeast cells with no ubiquitinylated H2B. Quantitative PCR was performed with primers for the coding region of an active gene. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR

Mouse Mono [C.15200232], 50 ug
Hu, Yt
ChIP, IP, WB

Histone H2B antibody, NBP2-59149, Novus Biologicals, Immunoprecipitation image

IP,

Histone H2B antibody, NBP2-59149, Novus Biologicals, Western Blot image

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 0.5 µg of Acetyl-Histone H2B (Lys12) polyclonal antibody (Product # PA5-40100). The IP'd DNA was subsequently analyzed on a HiSeq. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (fig 2A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding th

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Immunocytochemistry image

ICC,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies PA5-40103

Histone H2B antibody, PA5-40103, Invitrogen Antibodies. ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 2 µg of Acetyl-Histone H2B (Lys15) polyclonal antibody (Product # PA5-40103). The IP'd DNA was subsequently analyzed on a HiSeq. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (fig 2A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, IF, WB

AP,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Enzyme Linked Immunosorbent Assay image

ELISA,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Immunofluorescence image

IF,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Western Blot image

Supplier data
Cell Signaling Technology 34156S Histone H2B antibody, 34156S, Cell Signaling Technology.

Rabbit Mono [34156S], 100 ul
Bv, Ha, Hu, Rt
ChIP, FACS, ICC, IF, IHC-P, IP, WB

Histone H2B antibody, 34156S, Cell Signaling Technology, Flow Cytometry image

FACS,

Histone H2B antibody, 34156S, Cell Signaling Technology, Immunocytochemistry image

ICC,

Histone H2B antibody, 34156S, Cell Signaling Technology, Immunofluorescence image

IF,

Histone H2B antibody, 34156S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP, Histone H2B antibody, 34156S, Cell Signaling Technology, Western Blot image

Western Blot

Supplier data
Novus Biologicals NBP2-59270

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, IP, WB

Histone H2B antibody, NBP2-59270, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP, DB,

ELISA,

ICC,

IF,

Supplier data
Novus Biologicals NBP2-59271

Histone H2B antibody, NBP2-59271, Novus Biologicals. Western Blot: Histone H2B Antibody [NBP2-59271] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK12ac . The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

Histone H2B antibody, NBP2-59271, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP, DB,

ELISA,

Supplier data
Novus Biologicals NBP2-59272

Histone H2B antibody, NBP2-59272, Novus Biologicals. Western Blot: Histone H2B Antibody [NBP2-59272] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2BK15ac . The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

Histone H2B antibody, NBP2-59272, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP, DB,

ELISA,

Supplier data
Novus Biologicals NBP2-54770 Histone H2B antibody, NBP2-54770, Novus Biologicals. Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Histone H2B antibody, NBP2-54770, Novus Biologicals. Western Blot: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Mouse Mono [1B3F12/A9], 100 ul
Hu, Yt
ChIP, ELISA, IP, WB

Histone H2B antibody, NBP2-54770, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP,

ELISA,

Supplier data
Novus Biologicals NBP2-59149

Histone H2B antibody, NBP2-59149, Novus Biologicals. Western Blot: Histone H2B [ubiquitylated Lys123] Antibody (C.15200232) [NBP2-59149] - Histone H2B Antibody (C.15200232) [NBP2-59149] - Analysis on histone extracts from HeLa cells (15 ug) using the antibody against H2BK123ub. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.

Mouse Mono [C.15200232], 50 ug
Hu, Yt
ChIP, IP, WB

Histone H2B antibody, NBP2-59149, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP,

Supplier data
Invitrogen Antibodies PA5-40100

Histone H2B antibody, PA5-40100, Invitrogen Antibodies. Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), HeLa treated with sodium butyrate (5 nM for 24 hr) (Lane 2), COS-7 (Lane 3), and COS-7 treated with sodium butyrate (5 nM for 24 hr) (Lane 4). The blot was probed with Anti-Acetyl-Histone H2B (Lys12) polyclonal antibody (Product # PA5-40100, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 &micr

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, ICC, IF, WB

AP,

Histone H2B antibody, PA5-40100, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

ELISA,

ICC,

Supplier data
Invitrogen Antibodies PA5-40103

Histone H2B antibody, PA5-40103, Invitrogen Antibodies. Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the anti-H2BK15ac antibody (Product # PA5-40103). The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
AP, ChIP, ELISA, IF, WB

AP,

Histone H2B antibody, PA5-40103, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

ELISA,

Supplier data
Lifespan Biosciences LS-C353496 Histone H2B antibody, LS-C353496, Lifespan Biosciences. Western blot analysis of Histone H2B expression in U2OS (A); HeLa (B); A431 (C) whole cell lysates.

Histone H2B antibody, LS-C353496, Lifespan Biosciences. Western blot analysis of Histone H2B expression in U2OS (A); HeLa (B); A431 (C) whole cell lysates.

Rabbit Poly, 1 Vial
Bv, Ch, Hu, Mo, Pr, Zf
ChIP, ICC, IF, IHC-F, IHC-P, WB

ChIP, ICC, Histone H2B antibody, LS-C353496, Lifespan Biosciences, Immunofluorescence image

IF,

Histone H2B antibody, LS-C353496, Lifespan Biosciences, Immunohistochemistry frozen image

IHC-F,

Histone H2B antibody, LS-C353496, Lifespan Biosciences, Immunohistochemistry paraffin image

IHC-P

Supplier data
Cell Signaling Technology 34156S Histone H2B antibody, 34156S, Cell Signaling Technology.

Rabbit Mono [34156S], 100 ul
Bv, Ha, Hu, Rt
ChIP, FACS, ICC, IF, IHC-P, IP, WB

Histone H2B antibody, 34156S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

FACS,

ICC,

IF,

IHC-P, IP

Immunohistochemistry paraffin

Supplier data
Lifespan Biosciences LS-C353496 Histone H2B antibody, LS-C353496, Lifespan Biosciences. Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then i ...

Histone H2B antibody, LS-C353496, Lifespan Biosciences. Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then i ...

Rabbit Poly, 1 Vial
Bv, Ch, Hu, Mo, Pr, Zf
ChIP, ICC, IF, IHC-F, IHC-P, WB

ChIP, ICC, Histone H2B antibody, LS-C353496, Lifespan Biosciences, Immunofluorescence image

IF,

IHC-F,

Histone H2B antibody, LS-C353496, Lifespan Biosciences, Western Blot image

Supplier data
Cell Signaling Technology 34156S Histone H2B antibody, 34156S, Cell Signaling Technology.

Rabbit Mono [34156S], 100 ul
Bv, Ha, Hu, Rt
ChIP, FACS, ICC, IF, IHC-P, IP, WB

ChIP,

FACS,

ICC,

IF, IP,

Enzyme Linked Immunosorbent Assay

Supplier data
Novus Biologicals NBP2-59270 Histone H2B antibody, NBP2-59270, Novus Biologicals. ELISA: Histone H2B Antibody [NBP2-59270] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2B in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.

Histone H2B antibody, NBP2-59270, Novus Biologicals. ELISA: Histone H2B Antibody [NBP2-59270] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2B in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, ICC, IF, IP, WB

ChIP, DB,

ICC,

IF,

IP,

Supplier data
Novus Biologicals NBP2-59271

Histone H2B antibody, NBP2-59271, Novus Biologicals. ELISA: Histone H2B Antibody [NBP2-59271] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK12ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:38,200.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

ChIP, DB,

IF,

Supplier data
Novus Biologicals NBP2-59272

Histone H2B antibody, NBP2-59272, Novus Biologicals. ELISA: Histone H2B Antibody [NBP2-59272] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H2BK15ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:29,700.

Rabbit Poly, 50 ug
Hu
ChIP, DB, ELISA, IF, WB

ChIP, DB,

IF,

Supplier data
Novus Biologicals NBP2-54770 Histone H2B antibody, NBP2-54770, Novus Biologicals. ELISA: Histone H2B Antibody (1B3F12/A9) [NBP2-54770]

Mouse Mono [1B3F12/A9], 100 ul
Hu, Yt
ChIP, ELISA, IP, WB

ChIP,

IP,

Histone H2B antibodies are often published with	Associated citations
Histone H3	10.1%
Histone Cluster 3 H3	10.1%
Erk1	6.1%
Histone Cluster 1 H2A Family Member M	5.1%
GAPDH	5.1%
Synaptotagmin 1	4.0%
p65	4.0%
c-Myc	4.0%
BAX	4.0%
Histone Cluster 1 H2B Family Member J	3.0%

Histone H2B antibodies are often published with

Associated citations

Histone H3

10.1%

Histone Cluster 3 H3

10.1%

Erk1

6.1%

Histone Cluster 1 H2A Family Member M

5.1%

5.1%

4.0%

4.0%

4.0%

4.0%

Histone Cluster 1 H2B Family Member J

3.0%

All None
	WB	ELISA	IHC-P	IF	ICC	IHC-F	IP	AP	ChIP	FACS	DB	Pep-ELISA
Human	388	180	155	130	93	87	41	25	21	11	11	7
Mouse	262	107	142	111	74	78	34	4	12	7	7	7
Rat	198	45	77	54	53	31	33	4	10	8	7	7
Bovine	55	13	27	21	14	14	20		10	1	7
Primate	47	7	19	35	28	14	16	1	10	2	7
Chicken	39	15	25	8	6	12	8		8		7
Canine	26	2	14	22	20	9	7		6	1	7
Yeast	26	14		2			10		9		7
Zebrafish	22		8	7	5	7	9		9		7
Drosophila	19		4	2		3	7		8		7
Xenopus	16	2	2	2			8		7		7
Porcine	15	1	1	5	2		10		8		7
Equine	15	1	1	4	2		10		8		7
Hamster	15		1	3	2		9		7	2	7
Rabbit	14	2	3	2		1	7		6		7
Mammals	13		2	2		1	7		6		7
Eukaryotes	12			2			7		6		7
Guinea Pig	11			2			7		6		7
Feline	11		1	9	7		7		6	1	7
Fish	11	1		2	1		7		6		7
Bacteria	11		1	2		1	7		6		7
Plant	10			2			7		6		7
Prokaryotes	10			2			7		6		7
Donkey	10			2			7		6		7
Goat	10			2			7		6		7
C.Elegans	10			2			7		6		7
Sheep	10			2			7		6		7
Virus	10			2			7		6		7
Frog	10			2			7		6		7
Goldfish	10			2			7		6		7
Duck		5
Vertebrates	1	1		1	1
Bat	1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

13 matching Histone H2B antibodies for Chromatin Immunoprecipitation from 17 suppliers

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13 matching Histone H2B antibodies for Chromatin Immunoprecipitation after applying filters

Chromatin Immunoprecipitation - Use Filter to switch application

Western Blot

Immunohistochemistry paraffin

Enzyme Linked Immunosorbent Assay

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