POU Class 5 Homeobox 1 Antibodies for Chromatin Immunoprecipitation

6 matching POU Class 5 Homeobox 1 antibodies for Chromatin Immunoprecipitation after applying filters

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Chromatin Immunoprecipitation - Use Filter to switch application

Supplier data
Novus Biologicals NBP2-59164

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals. Chromatin Immunoprecipitation: OCT4 Antibody [NBP2-59164] - ChIP assays were performed using E14Tg2a mouse embryonic stem cells, the antibody against OCT4 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. Quantitative PCR was performed with primers for known OCT4 targets UTF1, YES1 and ZSCAN10, used as

Rabbit Poly, 50 ug
Hu
ChIP, ELISA, WB

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals, Enzyme Linked Immunosorbent Assay image

ELISA,

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals, Western Blot image

Supplier data
R&D Systems AF1759 POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems. Detection of Oct-3/4-regulated Genes by Chromatin Immunoprecipitation.

POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems. Detection of Oct-3/4-regulated Genes by Chromatin Immunoprecipitation.

Goat Poly, 100 ug
Hu, Mo
ChIP, ICC, IP, WB

POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems, Immunocytochemistry image

ICC, IP,

POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems, Western Blot image

Supplier data
Invitrogen Antibodies PA5-27194

POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies. Chromatin immunoprecipitation analysis of OCT3, 4 was performed using cross-linked chromatin from 1x10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of an OCT3, 4 polyclonal antibody (Product # PA5-27194). Chromatin aliquots from ~1x10^5 cells were used per Ch

Rabbit Poly, 100 ul
Hu
ChIP, FACS, ICC, IF, WB

POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 701756 POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies. Enrichment of endogenous OCT4 protein at specific gene loci using ABfinity

POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies. Enrichment of endogenous OCT4 protein at specific gene loci using ABfinity

Rabbit Mono [3H8L6], 100 ug
Hu, Mo
ChIP, FACS, ICC, IF, WB

POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 710788 POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies. Enrichment of endogenous OCT4 protein at specific gene loci using ABfinity

POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies. Enrichment of endogenous OCT4 protein at specific gene loci using ABfinity

Rabbit Poly, 100 ug
Hu
ChIP, ICC, IF, WB

ICC,

POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies, Western Blot image

Supplier data
Cell Signaling Technology 83932S POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology.

Rabbit Mono [83932S], 100 ul
Ha
ChIP, FACS, ICC, IF, IP, WB

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Flow Cytometry image

FACS,

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Immunocytochemistry image

ICC,

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Immunoprecipitation image

IP,

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 2750S POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology.

Rabbit Poly, 100 ul
Hu, Pr
ChIP, FACS, ICC, IF, IHC-P, WB

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Flow Cytometry image

FACS,

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Immunocytochemistry image

ICC,

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 2890S POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology.

Rabbit Mono [C52G3], 100 ul
Hu
ChIP, FACS, ICC, IF, IHC-P, WB

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Flow Cytometry image

FACS,

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Immunocytochemistry image

ICC,

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 83932T POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology.

Rabbit Mono [83932T], 20 ul
Ha
AP, ChIP, FACS, ICC, IF, IP, WB

AP,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Flow Cytometry image

FACS,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Immunocytochemistry image

ICC,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Immunofluorescence image

IF,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Immunoprecipitation image

IP,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 5677S POU Class 5 Homeobox 1 antibody, 5677S, Cell Signaling Technology.

Rabbit Mono [C30A3], 100 ul
Hu, Mo
ChIP

Western Blot

Supplier data
Novus Biologicals NBP2-67748 POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals. Western Blot: Oct-3/4 Antibody (SD0750) [NBP2-67748] - Analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: F9 Lane 2: NCCIT Lane 3: hES

Rabbit Mono [SD0750], 100 ul
Hu, Mo
ChIP, ICC, IF, IHC-F, IHC-P, IP, WB

ChIP,

POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals, Immunocytochemistry image

ICC,

POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals, Immunofluorescence image

IF, IHC-F, POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Novus Biologicals NBP2-59164 POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals. Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1;000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals. Western Blot: OCT4 Antibody [NBP2-59164] - Nuclear extracts (15 ug) from human embryonic stem cells were analysed by Western blot using the antibody against OCT4 diluted 1:1;000 in TBS- Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Rabbit Poly, 50 ug
Hu
ChIP, ELISA, WB

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals, Chromatin Immunoprecipitation image

ChIP,

ELISA

Supplier data
R&D Systems AF1759 POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems. Detection of Human Oct-3/4 by Simple Western<sup>TM</sup>.

Goat Poly, 100 ug
Hu, Mo
ChIP, ICC, IP, WB

POU Class 5 Homeobox 1 antibody, AF1759, R&D Systems, Chromatin Immunoprecipitation image

ChIP,

ICC, IP

Supplier data
Boster Biological Technology M00174 POU Class 5 Homeobox 1 antibody, M00174, Boster Biological Technology. Western blot analysis of Oct4 expression in NCCIT cell lysate.

POU Class 5 Homeobox 1 antibody, M00174, Boster Biological Technology. Western blot analysis of Oct4 expression in NCCIT cell lysate.

Rabbit Mono [M00174], 100 ug
Hu, Mo
ChIP, ICC, IF, IHC-F, IHC-P, IP, WB

ChIP, ICC, POU Class 5 Homeobox 1 antibody, M00174, Boster Biological Technology, Immunofluorescence image

IF, IHC-F, POU Class 5 Homeobox 1 antibody, M00174, Boster Biological Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Invitrogen Antibodies PA5-27194 POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies. Western blot analysis of OCT3, 4 using 20 µg of human ESC lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an OCT3, 4 polyclonal antibody (Product # PA5-27194) at a dilution of 1:500.

POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies. Western blot analysis of OCT3, 4 using 20 µg of human ESC lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an OCT3, 4 polyclonal antibody (Product # PA5-27194) at a dilution of 1:500.

Rabbit Poly, 100 ul
Hu
ChIP, FACS, ICC, IF, WB

POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

FACS, ICC, POU Class 5 Homeobox 1 antibody, PA5-27194, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies 701756

POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies. Knockdown of OCT4 was achieved by transfecting NTERA-2 cells with OCT4 specific siRNA (Silencer® select Product # s10871, Product # s10873). Western blot analysis (Fig a) was performed using Whole cell extract from the OCT4 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ABfinity™ Anti-OCT4 Rabbit Oligoclonal Antibody (Product# 710788, 2 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclona

Rabbit Mono [3H8L6], 100 ug
Hu, Mo
ChIP, FACS, ICC, IF, WB

POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

FACS, ICC, POU Class 5 Homeobox 1 antibody, 701756, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies 710788

POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies. Knockdown of OCT4 was achieved by transfecting NTERA-2 cells with OCT4 specific siRNA (Silencer® select Cat # s10871, Product # s10873). Western blot analysis (Fig a) was performed using Whole cell extract from the OCT4 knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ABfinity™ Anti-OCT4 Rabbit Oligoclonal Antibody (Product # 710788, 2 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal&t

Rabbit Poly, 100 ug
Hu
ChIP, ICC, IF, WB

POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP, ICC, POU Class 5 Homeobox 1 antibody, 710788, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Cell Signaling Technology 83932S POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology.

Rabbit Mono [83932S], 100 ul
Ha
ChIP, FACS, ICC, IF, IP, WB

POU Class 5 Homeobox 1 antibody, 83932S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

FACS,

ICC,

IF,

Supplier data
Cell Signaling Technology 2750S POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology.

Rabbit Poly, 100 ul
Hu, Pr
ChIP, FACS, ICC, IF, IHC-P, WB

POU Class 5 Homeobox 1 antibody, 2750S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

FACS,

ICC,

IF,

IHC-P

Supplier data
Cell Signaling Technology 2890S POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology.

Rabbit Mono [C52G3], 100 ul
Hu
ChIP, FACS, ICC, IF, IHC-P, WB

POU Class 5 Homeobox 1 antibody, 2890S, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

FACS,

ICC,

IF,

IHC-P

Supplier data
Cell Signaling Technology 83932T POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology.

Rabbit Mono [83932T], 20 ul
Ha
AP, ChIP, FACS, ICC, IF, IP, WB

AP,

POU Class 5 Homeobox 1 antibody, 83932T, Cell Signaling Technology, Chromatin Immunoprecipitation image

ChIP,

FACS,

ICC,

IF,

Enzyme Linked Immunosorbent Assay

Supplier data
Novus Biologicals NBP2-59164

POU Class 5 Homeobox 1 antibody, NBP2-59164, Novus Biologicals. ELISA: OCT4 Antibody [NBP2-59164] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against OCT4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:100,000.

Rabbit Poly, 50 ug
Hu
ChIP, ELISA, WB

ChIP,

Immunohistochemistry paraffin

Supplier data
Novus Biologicals NBP2-67748 POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals. Immunohistochemistry-Paraffin: Oct-3/4 Antibody (SD0750) [NBP2-67748] - Analysis of paraffin-embedded mouse liver tissue using anti-Oct4 antibody. Counter stained with hematoxylin.

Rabbit Mono [SD0750], 100 ul
Hu, Mo
ChIP, ICC, IF, IHC-F, IHC-P, IP, WB

ChIP,

ICC,

IF, IHC-F, IP, POU Class 5 Homeobox 1 antibody, NBP2-67748, Novus Biologicals, Western Blot image

POU Class 5 Homeobox 1 antibodies are often published with	Associated citations
Nanog Homeobox	27.4%
Quiescin Sulfhydryl Oxidase 1	21.6%
Stage-specific embryonic antigen 4	10.0%
Keratan sulfate antigen TRA1-60	7.9%
Nestin	6.8%
CD44	6.4%
Heat Shock Protein 90 Beta Family Member 1	5.8%
Keratan sulfate antigen TRA1-81	5.3%
Prominin 1	5.1%
Kruppel Like Factor 4	4.5%

POU Class 5 Homeobox 1 antibodies are often published with

Associated citations

Nanog Homeobox

27.4%

Quiescin Sulfhydryl Oxidase 1

21.6%

Stage-specific embryonic antigen 4

10.0%

Keratan sulfate antigen TRA1-60

7.9%

Nestin

6.8%

CD44

6.4%

Heat Shock Protein 90 Beta Family Member 1

5.8%

Keratan sulfate antigen TRA1-81

5.3%

Prominin 1

5.1%

Kruppel Like Factor 4

4.5%

All None
	WB	ELISA	IF	FACS	IHC-P	ICC	IHC-F	AP	IP	DB	ChIP	Pep-ELISA	EMSA
Human	478	233	208	204	164	157	118	40	39	24	15	7
Mouse	247	76	108	113	88	94	57	7	32		8	7	2
Rat	153	73	65	38	82	36	54	2	14			7
Primate	35	5	16	17	4	16	2				2
Hamster	26	18	7	7	18	6	18	1	4		3
Bovine	25	4	9	8	9	9	7		2
Canine	24	9	7	5	9	7	4					2
Porcine	13	2	2	2	5	2	5		2
Rabbit	13	4	8	6	5	5	4		3
Zebrafish	11	5	1	1	1	1	1
Guinea Pig	9	1	1	1	5	1	4
Sheep	6		4	4		4
Goat	6	1			2		2
Bacteria	4		1	1	2	1	2
Equine	3	2
Frog	1		1	1		1			1
Plant	1	1
Invertebrates			1	1		1
Chicken			1	1		1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

6 matching POU Class 5 Homeobox 1 antibodies for Chromatin Immunoprecipitation from 28 suppliers

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POU Class 5 Homeobox 1 information

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1 Applications, 19 Species currently selected

6 matching POU Class 5 Homeobox 1 antibodies for Chromatin Immunoprecipitation after applying filters

Chromatin Immunoprecipitation - Use Filter to switch application

Western Blot

Enzyme Linked Immunosorbent Assay

Immunohistochemistry paraffin

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