For over two decades, Aves Labs has been producing custom chicken polyclonal antibodies for customers in academia and industry worldwide.
Our Process Produces Antibodies That Work
Over our long experience in manufacturing custom chicken polyclonal antibodies for diverse customers in academia and the biotech, diagnostic and pharmaceutical industries, we have developed a number of proprietary methods and procedures that optimize the immune response of our host animals and improve the quality of our products. Whereas most competitors work primarily with rabbits, our scientists' deep experience with chickens allows our customers to benefit from unique capabilities, among them improved algorithms for analyzing protein sequences to identify immunogenic peptides.
We Can Support Projects of Any Size
Custom chicken antibodies can require two hens or two hundred hens, which is why we designed our new facilities with flexibility in mind. As a result, Aves can tailor our custom antibodies service to suit your needs, including project sizes up to "bulk" production levels (>100mg).
Service Packages
Our service comes in several packages (prices do not include shipping and handling). Contact us with you project details. We look forward to providing you with a speedy project consultation and quote.
Package #1:
Custom Polyclonal Antibody Production
Timeframe:
11-week production protocol
Price:
$1,250 + Shipping and Handling
Antibodies generated against a customer-supplied antigen. Includes:
- Purified immune IgY from two (2) hens (~800 mg IgY / hen)
- Pre-immune IgY from each hen (~150 mg of IgY / hen)
- Four (4) Immunizations per hen
Package #2:
Custom Peptide Antibody Production
Timeframe:
16- to 18-week production protocol
Price:
$3,200 + Shipping and Handling
Affinity-purified antibodies generated against a synthesized peptide. Includes:
- Peptide design
- Peptide synthesis up to twenty (20) residues
- Conjugation to KLH
- Affinity purification pooled from both hens
- ELISA analysis
- Pre-immune IgY from each hen (~150 mg IgY / hen)
- Remaining peptides (~20 mg)
Package #3:
Custom Phosphospecific Antibody Production
Timeframe:
16- to 18-week production protocol
Price:
Contact us for a quote.
Affinity purified antibodies against a phosphospecific peptide. Includes:
- Peptide synthesis of non-phosphorylated peptide
- Peptide synthesis of phosphorylated peptide
- Conjugation to KLH
- Positive affinity purification
- Negative affinity purification
- ELISA analysis
- Remaining peptides (~20 mg)
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Service Options
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Antibody Purity
We can provide either an "IgY fraction" (i.e., a purified preparation of IgY in PBS, but containing only a small fraction against the antigen of interest) or "Affinity Purified Antibodies" using an affinity matrix (in which the entire antibody recognizes the antigen). For some applications (e.g., Western Blot, immunocytochemistry, etc.), the IgY fraction may be sufficient. For other applications (e.g., immunoprecipitation, etc.), affinity purified antibody is recommended. -
Number of Hens
Since chickens are outbred species (like rabbits), we highly recommended injecting at least two hens. From that starting point, the number of hens to be injected depends on the amount of antibody you require as well as the hens' inherent immunogenicity. -
ELISA
We can perform an ELISA assay, which provides information about antibody titres and antibody avidities for the antigen. This information can help provide guidance about antibody dilutions in your studies. -
Additional Antibodies
After delivering antibody preparations to you, we can continue to collect and store approximately twelve (12) immune eggs for a period of two (2) months. These eggs can be used to prepare additional batches of antibodies at a significantly reduced cost. You may also choose to have us continue boosting the hens to collect additional batches of eggs.
