AKT1 Substrate 1 Monoclonal Antibodies

AKT1 Substrate 1 monoclonal antibodies

63 AKT1 Substrate 1 monoclonal antibodies from 5 antibody suppliers Click each AKT1 Substrate 1 monoclonal antibody that interests you to view the full AKT1 Substrate 1 monoclonal antibody datasheet.

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Supplier data
Bio-Rad (formerly AbD Serotec) VMA00493

AKT1 Substrate 1 antibody, VMA00493, Bio-Rad (formerly AbD Serotec) . Western blot analysis of whole cell lysates probed with PRAS40 antibody followed by detection with HRP conjugated Goat anti Mouse IgG antibody (1/10,000, <a href="https://www.bio-rad-antibodies.com/product/partnumber/STAR207P.html">STAR207P</a>) and visualized on the <a rel="nofollow" href="http://www.bio-rad.com/en-us/product/chemidoc-imaging-systems/chemidoc-mp-system"target="_blank">ChemiDoc™ MP</a> with 18 second exposure. Arrow points to PRAS40 (molecular weight 40 kDa)

Mouse Mono [VMA00493], 100 ul

WB

Supplier data
R&D Systems MAB6890

AKT1 Substrate 1 antibody, MAB6890, R&D Systems. Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # <A class=NoLineLink href="http://www.rndsystems.com/search?keywords=291-G1">291-G1</A>) for 20 minutes and 10 ng/mL Recombinant Human PDGF-BB (Catalog # <A class=NoLineLink href="http://www.rndsystems.com/search?keywords=220-BB">220-BB</A

Mouse Mono [760502], 100 ug
Hu, Mo
ICC, IF, WB

AKT1 Substrate 1 antibody, MAB6890, R&D Systems, Immunocytochemistry image

ICC,

AKT1 Substrate 1 antibody, MAB6890, R&D Systems, Immunofluorescence image

Supplier data
R&D Systems MAB6408 AKT1 Substrate 1 antibody, MAB6408, R&D Systems. Detection of Human PRAS40 by Simple Western<SUP>TM</SUP>.

Mouse Mono [660928], 100 ug
Hu
ICC, IF, WB

AKT1 Substrate 1 antibody, MAB6408, R&D Systems, Immunocytochemistry image

ICC,

AKT1 Substrate 1 antibody, MAB6408, R&D Systems, Immunofluorescence image

Supplier data
R&D Systems MAB68901

AKT1 Substrate 1 antibody, MAB68901, R&D Systems. Detection of Human and Mouse Phospho-PRAS40 (T246) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/ml Recombinant Human IGF-I (Catalog # <a class="NoLineLink" href="http://www.rndsystems.com/product_results.aspx?k=291-G1">291-G1</a>) for 60 minutes and 100 ng/ml Recombinant Human PDGF-BB (Catalog # <a class="NoLineLink" href="http://www.rndsystems.com/product_results.aspx?

Rabbit Mono [1211A], 100 ul
Hu
WB

Supplier data
Boster Biological Technology M03629 AKT1 Substrate 1 antibody, M03629, Boster Biological Technology. Western blot analysis of PRAS40 expression in 293T cell lysate (M03629) in 293T cell lysate. <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis

AKT1 Substrate 1 antibody, M03629, Boster Biological Technology. Western blot analysis of PRAS40 expression in 293T cell lysate (M03629) in 293T cell lysate. <br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br> After Electrophoresis

Rabbit Mono [M03629], 100 ug
Hu, Mo
FACS, IHC-F, IHC-P, IP, WB

FACS, IHC-F, IHC-P, IP

Supplier data
Boster Biological Technology M03629-1

AKT1 Substrate 1 antibody, M03629-1, Boster Biological Technology. Figure 1. Western blot analysis of AKT1S1 using anti-AKT1S1 antibody (M03629-1) in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.<br>Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. <br>After Electrophoresis

Rabbit Mono [M03629-1], 100 ug
Hu, Mo, Rt
IP, WB

Supplier data
Invitrogen Antibodies AHO1031

AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies. Extract prepared from human HeLa cells was resolved by SDS-PAGE on a 4-20% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% milk-TBST buffer and then incubated with this PRAS40 monoclonal antibody (clone 73P21) at a concentration of 0.50 µg/mL in a 5% milk-TBST buffer for two hours at room temperature. After washing, the membrane was incubated with goat F (ab

Mouse Mono [73P21], 100 ug
Hu
ICC, IF, IHC-F, IHC-P, IP, WB

ICC,

AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies, Immunofluorescence image

IF,

AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies, Immunohistochemistry frozen image

IHC-F,

AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Invitrogen Antibodies 701058

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies. Western blot analysis of Phospho-AKT1S1 pThr246 in whole cell extracts of HepG2 cells treated with PDGF (100 ng/mL, 15 min) using a Phospho-AKT1S1 pThr246 recombinant rabbit monoclonal antibody (Product # 701058) at a dilution of 2.5 µg/mL. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 1). Results show a band at ~40 kDa.

Rabbit Mono [701058], 100 ug
Hu
FACS, IF, WB

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies, Flow Cytometry image

FACS,

AKT1 Substrate 1 antibody, 701058, Invitrogen Antibodies, Immunofluorescence image

Supplier data
Invitrogen Antibodies 701075 AKT1 Substrate 1 antibody, 701075, Invitrogen Antibodies. Western blot analysis of AKT1S1 in whole cell extracts of HeLa (lane 1) and A431 (lane 2) using an AKT1S1 recombinant rabbit monoclonal antibody (Product # 701075) at a dilution of 2.5

AKT1 Substrate 1 antibody, 701075, Invitrogen Antibodies. Western blot analysis of AKT1S1 in whole cell extracts of HeLa (lane 1) and A431 (lane 2) using an AKT1S1 recombinant rabbit monoclonal antibody (Product # 701075) at a dilution of 2.5

Rabbit Mono [701075], 100 ug
Hu
IF, WB

AKT1 Substrate 1 antibody, 701075, Invitrogen Antibodies, Immunofluorescence image

Supplier data
NSJ Bioreagents F40342-0.4ML AKT1 Substrate 1 antibody, F40342-0.4ML, NSJ Bioreagents. AKT1S1 antibody western blot analysis in A375 lysate

AKT1 Substrate 1 antibody, F40342-0.4ML, NSJ Bioreagents. AKT1S1 antibody western blot analysis in A375 lysate

Mouse Mono [449CT13.4.2], 400 ul
Hu
ELISA, WB

ELISA

Supplier data
NSJ Bioreagents F53618-0.1ML AKT1 Substrate 1 antibody, F53618-0.1ML, NSJ Bioreagents. AKT1S1 antibody western blot analysis in A375 lysate

AKT1 Substrate 1 antibody, F53618-0.1ML, NSJ Bioreagents. AKT1S1 antibody western blot analysis in A375 lysate

Mouse Mono [449CT13.4.2], 100 ul
Hu
ELISA, WB

ELISA

Supplier data
Cell Signaling Technology 9614L AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology.

Rabbit Mono [110B7E], 300 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
ELISA, IHC-P, IP, WB

AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology, Enzyme Linked Immunosorbent Assay image

ELISA,

AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P,

AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology, Immunoprecipitation image

Supplier data
Cell Signaling Technology 2997T AKT1 Substrate 1 antibody, 2997T, Cell Signaling Technology.

Rabbit Mono [2997T], 20 ul
Ha, Hu, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2997T, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 2691T AKT1 Substrate 1 antibody, 2691T, Cell Signaling Technology.

Rabbit Mono [2691T], 20 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2691T, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 13175S AKT1 Substrate 1 antibody, 13175S, Cell Signaling Technology.

Rabbit Mono [D4D2], 100 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 13175S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 2691P AKT1 Substrate 1 antibody, 2691P, Cell Signaling Technology.

Rabbit Mono [D23C7], 40 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2691P, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 2691S AKT1 Substrate 1 antibody, 2691S, Cell Signaling Technology.

Rabbit Mono [D23C7], 100 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2691S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 13175T AKT1 Substrate 1 antibody, 13175T, Cell Signaling Technology.

Rabbit Mono [13175T], 20 ul
Ha, Hu, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 13175T, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 13175P AKT1 Substrate 1 antibody, 13175P, Cell Signaling Technology.

Rabbit Mono [D4D2], 40 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 13175P, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 2997S AKT1 Substrate 1 antibody, 2997S, Cell Signaling Technology.

Rabbit Mono [C77D7], 100 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2997S, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 2997P AKT1 Substrate 1 antibody, 2997P, Cell Signaling Technology.

Rabbit Mono [C77D7], 40 ul
Hu, Mo, Pr, Rt
IHC-P, IP, WB

AKT1 Substrate 1 antibody, 2997P, Cell Signaling Technology, Immunohistochemistry paraffin image

IHC-P, IP

Supplier data
Cell Signaling Technology 8858S AKT1 Substrate 1 antibody, 8858S, Cell Signaling Technology.

Rabbit Mono [D23C7], 100 ul
Ha, Hu, Pr, Rt
WB

Supplier data
Cell Signaling Technology 6950S AKT1 Substrate 1 antibody, 6950S, Cell Signaling Technology.

Rabbit Mono [110B7E], 100 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
WB

Supplier data
Abbexa abx025386 AKT1 Substrate 1 antibody, abx025386, Abbexa.

Mouse Mono [abx025386], 100 ug
Hu
WB

Immunoprecipitation

Supplier data
Cell Signaling Technology 9614L AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology.

Rabbit Mono [110B7E], 300 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
ELISA, IHC-P, IP, WB

ELISA,

IHC-P,

AKT1 Substrate 1 antibody, 9614L, Cell Signaling Technology, Western Blot image

Supplier data
Cell Signaling Technology 8050S AKT1 Substrate 1 antibody, 8050S, Cell Signaling Technology.

Rabbit Mono [110B7E], 400 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
IP

Supplier data
Cell Signaling Technology 9646S AKT1 Substrate 1 antibody, 9646S, Cell Signaling Technology.

Rabbit Mono [110B7E], 400 ul
Bac, Bv, Cel, Ch, Cn, Dk, Dr, Eq, Eu, Fe, Fi, Fr, Gf, GP, Gt, Ha, Hu, Mam, Mo, Pl, Po, Pr, Pro, Rb, Rt, Sh, Vi, Xe, Yt, Zf
IP

Immunohistochemistry paraffin

Supplier data
Lifespan Biosciences LS-B2541 AKT1 Substrate 1 antibody, LS-B2541, Lifespan Biosciences. Anti-AKT1S1 / PRAS40 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

AKT1 Substrate 1 antibody, LS-B2541, Lifespan Biosciences. Anti-AKT1S1 / PRAS40 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Mouse Mono [LS-B2541], 1 Vial
Hu
IHC-F, IHC-P, IP, WB

AKT1 Substrate 1 antibody, LS-B2541, Lifespan Biosciences, Immunohistochemistry frozen image

IHC-F, IP, WB

Supplier data
Invitrogen Antibodies AHO1031

AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies. Immunohistochemistry analysis of PRAS40 showing staining in the cytoplasm and nucleus of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PRAS40 monoclonal antibody (Pr

Mouse Mono [73P21], 100 ug
Hu
ICC, IF, IHC-F, IHC-P, IP, WB

ICC,

IF,

IHC-F, IP, AKT1 Substrate 1 antibody, AHO1031, Invitrogen Antibodies, Western Blot image

AKT1 Substrate 1 antibodies are often published with	Associated citations
Akt	41.7%
Distal-Less Homeobox 4	16.7%
Regulatory Associated Protein Of MTOR Complex 1	12.5%
Eukaryotic Translation Initiation Factor 4E Binding Protein 1	12.5%
GAPDH	12.5%
N-Myc Downstream Regulated 1	12.5%
Mechanistic Target Of Rapamycin Kinase	8.3%
Transcription Factor 3	8.3%
VPS39 Subunit Of HOPS Complex	8.3%
RPTOR Independent Companion Of MTOR Complex 2	8.3%

AKT1 Substrate 1 antibodies are often published with

Associated citations

Akt

41.7%

Distal-Less Homeobox 4

16.7%

Regulatory Associated Protein Of MTOR Complex 1

12.5%

Eukaryotic Translation Initiation Factor 4E Binding Protein 1

12.5%

GAPDH

12.5%

N-Myc Downstream Regulated 1

12.5%

Mechanistic Target Of Rapamycin Kinase

8.3%

Transcription Factor 3

8.3%

VPS39 Subunit Of HOPS Complex

8.3%

RPTOR Independent Companion Of MTOR Complex 2

8.3%

All None
	WB	IP	IHC-P	IF	ELISA	ICC	IHC-F	FACS
Human	24	16	13	5	3	3	3	2
Rat	13	13	10		1
Mouse	12	12	9	1	1	1	1	1
Primate	12	12	10		1
Hamster	5	5	3		1
Yeast	2	3	1		1
C.Elegans	2	3	1		1
Mammals	2	3	1		1
Rabbit	2	3	1		1
Equine	2	3	1		1
Xenopus	2	3	1		1
Bovine	2	3	1		1
Frog	2	3	1		1
Virus	2	3	1		1
Drosophila	2	3	1		1
Goldfish	2	3	1		1
Bacteria	2	3	1		1
Eukaryotes	2	3	1		1
Canine	2	3	1		1
Sheep	2	3	1		1
Zebrafish	2	3	1		1
Feline	2	3	1		1
Goat	2	3	1		1
Prokaryotes	2	3	1		1
Chicken	2	3	1		1
Donkey	2	3	1		1
Plant	2	3	1		1
Porcine	2	3	1		1
Guinea Pig	2	3	1		1
Fish	2	3	1		1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

63 matching AKT1 Substrate 1 monoclonal antibodies from 5 suppliers

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AKT1 Substrate 1 information

Aliases

8 Applications, 30 Species currently selected