Mechanistic Target Of Rapamycin Kinase Antibodies for Chromatin Immunoprecipitation

0 matching Mechanistic Target Of Rapamycin Kinase antibodies for Chromatin Immunoprecipitation after applying filters

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Chromatin Immunoprecipitation - Use Filter to switch application

Supplier data
Invitrogen Antibodies PA5-34663 Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Cross-linked ChIP was performed with 293T chromatin extract and 5 µg of either control rabbit IgG or a mTOR polyclonal antibody (Product # PA5-34663). The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter.

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Cross-linked ChIP was performed with 293T chromatin extract and 5 µg of either control rabbit IgG or a mTOR polyclonal antibody (Product # PA5-34663). The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, IF, IHC-F, IHC-P, IP, WB

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Immunofluorescence image

IF,

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Immunohistochemistry frozen image

IHC-F,

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Immunohistochemistry paraffin image

IHC-P,

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Immunoprecipitation image

IP,

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Western Blot image

Supplier data
Invitrogen Antibodies 710408

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies. Chromatin immunoprecipitation analysis of mTOR was performed using cross-linked chromatin from 1 x 10^6 HTC-IR rat hepatoma cells treated with insulin for 0, 5, and 30 minutes. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (see reference for Matrix ChIP protocol: http://www.ncbi.nlm.nih.gov/pubmed/22098709) with 1.0 µL/100 µL well volume of an mTOR rabbit oligoclonal antibody (Product # 710408). Chromatin aliquots from ~1 x 10^5 cells were used per

Rabbit Poly, 100 ug
Hu
ChIP, FACS, ICC, IF, WB

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Flow Cytometry image

FACS, ICC, Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Immunofluorescence image

IF,

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Western Blot image

Supplier data
GeneTex GTX101557 Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. Cross-linked ChIP was performed with 293T chromatin extract and 5 μg of either control rabbit IgG or anti-mTOR antibody. The precipitated DNA was detected by PCR with primer set targeting to 45S rDNA promoter .

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, ICC, IF, IHC-P, IP, WB

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex, Immunocytochemistry image

ICC,

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex, Immunofluorescence image

IF,

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex, Immunohistochemistry paraffin image

IHC-P,

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex, Immunoprecipitation image

IP,

Western Blot

Supplier data
Enzo Life Sciences ALX-215-065-1 Mechanistic Target Of Rapamycin Kinase antibody, ALX-215-065-1, Enzo Life Sciences. WB using

Rabbit Poly, 1 Vial
Hu
ChIP, ICC, WB

ChIP, ICC

Supplier data
Invitrogen Antibodies PA5-34663 Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Western blot analysis of mTOR using 50 µg mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a mTOR polyclonal antibody (Product # PA5-34663) at a dilution of 1:500.

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Western blot analysis of mTOR using 50 µg mouse brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a mTOR polyclonal antibody (Product # PA5-34663) at a dilution of 1:500.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, IF, IHC-F, IHC-P, IP, WB

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

IF,

IHC-F,

IHC-P,

Supplier data
Invitrogen Antibodies 710408 Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies. Western blot analysis of mTOR in whole COS7 and U87-MG lysate using a mTOR recombinant rabbit oligoclonal antibody (Product # 710408) at a dilution of 2

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies. Western blot analysis of mTOR in whole COS7 and U87-MG lysate using a mTOR recombinant rabbit oligoclonal antibody (Product # 710408) at a dilution of 2

Rabbit Poly, 100 ug
Hu
ChIP, FACS, ICC, IF, WB

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Chromatin Immunoprecipitation image

ChIP,

FACS, ICC, Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Immunofluorescence image

Supplier data
GeneTex GTX101557 Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR antibody [C3], C-term (GTX101557) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, ICC, IF, IHC-P, IP, WB

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex, Chromatin Immunoprecipitation image

ChIP,

ICC,

IF,

IHC-P,

Immunohistochemistry paraffin

Supplier data
Invitrogen Antibodies PA5-34663 Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Immunohistochemical analysis of mTOR in the cytosol of paraffin-embedded mouse prostate tissue using a mTOR polyclonal antibody (Product # PA5-34663) at a dilution of 1:500.

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Immunohistochemical analysis of mTOR in the cytosol of paraffin-embedded mouse prostate tissue using a mTOR polyclonal antibody (Product # PA5-34663) at a dilution of 1:500.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, IF, IHC-F, IHC-P, IP, WB

ChIP,

IF,

IHC-F,

IP,

Supplier data
GeneTex GTX101557 Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human colon cancer.mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.Antigen Retrieval: Citrate buffer, 20 min

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).</br>Sample: Paraffin-embedded human colon cancer.</br>mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, 20 min

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, ICC, IF, IHC-P, IP, WB

ChIP,

ICC,

IF,

IP,

Immunohistochemistry frozen

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, IF, IHC-F, IHC-P, IP, WB

ChIP,

IF,

IHC-P,

IP,

Immunofluorescence

Supplier data
Invitrogen Antibodies PA5-34663 Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Immunofluorescent analysis of mTOR showing staining in the cytoplasm and nucleus of MCF-7 cells. MCF-7 cells were fixed in 100% MeOH for 5 min and stained using a mTOR polyclonal antibody (Product # PA5-34663) diluted at 1:500. Blue: Hoechst 33342 staining.

Mechanistic Target Of Rapamycin Kinase antibody, PA5-34663, Invitrogen Antibodies. Immunofluorescent analysis of mTOR showing staining in the cytoplasm and nucleus of MCF-7 cells. MCF-7 cells were fixed in 100% MeOH for 5 min and stained using a mTOR polyclonal antibody (Product # PA5-34663) diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, IF, IHC-F, IHC-P, IP, WB

ChIP,

IHC-F,

IHC-P,

IP,

Supplier data
Invitrogen Antibodies 710408

Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies. Immunofluorescent analysis of mTOR in HeLa cells using a mTOR recombinant rabbit oligoclonal antibody (Product # 710408) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of mTOR.

Rabbit Poly, 100 ug
Hu
ChIP, FACS, ICC, IF, WB

ChIP,

FACS, ICC, Mechanistic Target Of Rapamycin Kinase antibody, 710408, Invitrogen Antibodies, Western Blot image

Supplier data
GeneTex GTX101557 Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10 μm.

Mechanistic Target Of Rapamycin Kinase antibody, GTX101557, GeneTex. mTOR antibody [C3], C-term detects mTOR protein at cytoplasm by immunofluorescent analysis.</br>Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.</br>Green: mTOR stained by mTOR antibody [C3], C-term (GTX101557) diluted at 1:2000.</br>Blue: Hoechst 33342 staining.</br>Scale bar= 10 μm.

Rabbit Poly, 100 ul
Hu, Mo, Rt
ChIP, ICC, IF, IHC-P, IP, WB

ChIP,

ICC,

IHC-P,

IP,

Mechanistic Target Of Rapamycin Kinase antibodies are often published with	Associated citations
Akt	15.1%
Erk1	4.2%
Ribosomal Protein S6 Kinase B1	3.8%
BCL2	3.4%
Beclin 1	3.2%
p38	3.2%
Protein Kinase AMP-Activated Catalytic Subunit Alpha 2	3.2%
Microtubule Associated Protein 1 Light Chain 3 Beta	3.2%
Protein Kinase AMP-Activated Non-Catalytic Subunit Beta 1	2.8%
GAPDH	2.6%

Mechanistic Target Of Rapamycin Kinase antibodies are often published with

Associated citations

Akt

15.1%

Erk1

4.2%

Ribosomal Protein S6 Kinase B1

3.8%

BCL2

3.4%

Beclin 1

3.2%

p38

3.2%

Protein Kinase AMP-Activated Catalytic Subunit Alpha 2

3.2%

Microtubule Associated Protein 1 Light Chain 3 Beta

3.2%

Protein Kinase AMP-Activated Non-Catalytic Subunit Beta 1

2.8%

GAPDH

2.6%

All None
	WB	IHC-P	IHC-F	IF	ELISA	ICC	IP	FACS	ChIP	Pep-ELISA	AP	PLA	DB
Human	284	147	120	72	65	49	26	19	4	2	1	1	1
Mouse	148	103	79	47	46	27	18	16	2	2	1
Rat	136	92	73	46	47	24	18	5	2	2	1	1
Primate	15	2		6		4	6	3			1
Sheep	10	9	3	5	5	5	2			2
Equine	9	6		4	4	2	3	3		2	1
Canine	4	5	1	1	5					2
Hamster	4			1	1	1	2
Rabbit	4	3	1		2
Guinea Pig	3	1		2	1	1	3			1
Bacteria	3			1		1	1
Bovine	1	2			2					1
Chicken	2	1	1	2		1	1				1
Porcine	2	1		1	1	1	1
Zebrafish	1	1	1	1	1

Bac	Bacteria
Bt	Bat
Bv	Bovine
Cel	C.Elegans
Cml	Camel
Cn	Canine
chem	Chemical
Ch	Chicken
Dk	Donkey
Dr	Drosophila
Du	Duck
Eq	Equine
Eu	Eukaryotes
Fe	Feline
Fi	Fish
Fr	Frog
Fu	Fungus
Gt	Goat
Gf	Goldfish
GP	Guinea Pig
Ha	Hamster
Hu	Human
Inv	Invertebrates
Llm	Llama
Mam	Mammals
Mo	Mouse
Pl	Plant
Po	Porcine
Pr	Primate
Pro	Prokaryotes
Rb	Rabbit
Rt	Rat
Sh	Sheep
Sq	Squirrel
Vert	Vertebrates
Vi	Virus
Xe	Xenopus
Yt	Yeast
Zf	Zebrafish

AP	Affinity purification
Bl	Blocking
ChIP	Chromatin Immunoprecipitation
DB	Dot Blot
EMSA	Electron Microscopy
ELISA	Enzyme Linked Immunosorbent Assay
FACS	Flow Cytometry
FA	Functional Assay
GS	Gel Shift
ICC	Immunocytochemistry
ID	Immunodiffusion
IF	Immunofluorescence
IHC-F	Immunohistochemistry frozen
IHC-P	Immunohistochemistry paraffin
IP	Immunoprecipitation
IVT	In vivo targeting
Neu	Neutralising
Pep-ELISA	Peptide ELISA
PLA	Proximity Ligation Assay
RIA	Radioimmunoassay
WB	Western Blot

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0 matching Mechanistic Target Of Rapamycin Kinase antibodies for Chromatin Immunoprecipitation after applying filters

Chromatin Immunoprecipitation - Use Filter to switch application

Western Blot

Immunohistochemistry paraffin

Immunohistochemistry frozen

Immunofluorescence

Immunocytochemistry

Immunoprecipitation

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